Development and validation of spectrophotometry uv-vis method for determination of primaquine and chloroquine in liposome dosage form

Development and validation of UV-Vis Spectrophotometry method for simultaneous determination of primaquine and chloroquine in liposome dosage form has been carried out. The method was tested for selectivity, linearity, accuracy, and precision. A phosphate buffer solution pH 7.4 was used as a solvent and observations were made at wavelengths of 220 and 260 nm for simultaneous equations method. The selectivity results of primaquine and chloroquine showed no interference from liposome. Linearity result (r value) of the simultaneous equation method was 0.9998 with the value of Vxo less than 0.5% for both primaquine and chloroquine in the concentration range of 2-10 mg/L. Accuracy was done using spiked placebo method and obtained data analyzed using simultaneous equation method. Persentage recovery of primaquine was 89-97% and 79-108% for chloroquine. The intra-and interday precision of primaquine were 1.72 and 2.57%, respectively. Whereas the intra-and interday precision of chloroquine were 6.93 and 8.77, respectively. Further observation using chromatography method need to be done to have better accuracy results for both substances.