Abstract
A high performance liquid chromatographic (HPLC) method for simultaneous determination of curcumin (C), demethoxycurcumin (DMC), and bis-demethoxycurcumin (BDMC) has been developed. Reversed phase C-18 column (Licrosphere) was used as a separation column with a mobile phase consisted of acetonitrile:1% trifluoroacetic acid (50:50) and flow rate of 1.0 ml/min. Elution was monitored at 422 nm. The method showed sensitivity, selectivity, precision and reproducibility at the selected HPLC conditions. Linear range was found from 60 to 200 μg/ml, with Vxo value less than 2%. The percent recoveries of curcuminoids were 110.61 ± 6.51, 107.69 ± 5.91 and 102.08 ± 4.89% of C, DMC and BDMC, respectively. Further, the method was used for determination of curcuminoids in herbal drinks containing curcuminoids.
| Original language | English |
|---|---|
| Pages (from-to) | 392-396 |
| Number of pages | 5 |
| Journal | International Journal of Pharmaceutical and Clinical Research |
| Volume | 8 |
| Issue number | 5 |
| Publication status | Published - 2016 |
Keywords
- Curcuminoids
- HPLC
- Herbal drinks
- Validation method