Effervescence-LPME is a new method that combines the use of effervescence and LPME techniques which are considered "green" chemistry methods. It has been used to determine the levels of chlorpyrifos compounds in cabbage, continued by analysis with high performance liquid chromatography (HPLC) with UV-Vis detector. The objectives of this research were to determine and optimize parameters of the effervescence-LPME method, namely type of organic solvent, sample volume and salt concentration, together with the determination of linearity, limit of detection, recovery, and enrichment factor of the effervescence-LPME method. The application of the effervescence-LPME as a sample preparation method in the analysis of chlorpyrifos in cabbage with HPLC was also studied. The results for the optimized analytical parameters of the effervescence-LPME method were identified as: n-hexane as the organic solvent, sample volume of 10 mL, without salt addition. The results of the validation of the method indicate that a linier calibration curve for chlorpyrifos standard solution 10 - 50 g/L has a correlation coefficient 0.9994, limit of detection (LOD) - 1.39 g/L, recovery - 97.80 - 101.97%, the coefficients of variation were within 0.18 - 2.25 % and the enrichment factor - 100.04. This method can be applied to determine chlorpyrifos in cabbage. It was established that with these optimal conditions chlorpyrifos levels in cabbage could not be obtained, because its concentration is beyond the limit of detection.
|Number of pages||6|
|Journal||Journal of Chemical Technology and Metallurgy|
|Publication status||Published - 2017|
- Effervescent-Liquid Phase Microextraction
- High Performance Liquid Chromatography