Deteksi Gen Reseptor Luteinizing Hormone (rLH) pada Sapi Madrasin dengan Metode Polymerase Chain Reaction

Ivo Febrina Prasetyo; Budi Utomo; Sri Pantja Madyawati

doi:10.20473/jmv.vol4.iss1.2021.125-130

Deteksi Gen Reseptor Luteinizing Hormone (rLH) pada Sapi Madrasin dengan Metode Polymerase Chain Reaction

Translated title of the contribution: Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction

Ivo Febrina Prasetyo, Budi Utomo, Sri Pantja Madyawati

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.

Translated title of the contribution	Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction
Original language	Indonesian
Pages (from-to)	125-130
Number of pages	6
Journal	Jurnal Medik Veteriner
Volume	4
Issue number	1
DOIs	https://doi.org/10.20473/jmv.vol4.iss1.2021.125-130
Publication status	Published - Apr 2021

Keywords

Madrasin cattle
PCR
gene
rLH

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title = "Deteksi Gen Reseptor Luteinizing Hormone (rLH) pada Sapi Madrasin dengan Metode Polymerase Chain Reaction",

abstract = "The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.",

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author = "Prasetyo, {Ivo Febrina} and Budi Utomo and Madyawati, {Sri Pantja}",

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AU - Prasetyo, Ivo Febrina

AU - Utomo, Budi

AU - Madyawati, Sri Pantja

PY - 2021/4

Y1 - 2021/4

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AB - The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.

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Deteksi Gen Reseptor Luteinizing Hormone (rLH) pada Sapi Madrasin dengan Metode Polymerase Chain Reaction

Abstract

Keywords

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