TY - GEN
T1 - Detection of microbial presence in the room, equipment, and cell culture media at temporary stem cell laboratory
AU - Rahayu, Tri
AU - Prasetya, Indra Adi Wira
AU - Triwahyuni, Desi
AU - Khiftiyah, Ana Mariatul
AU - Aini, Nurul
AU - Bachruddin, Muhammad
AU - Tsana, Izdihar
AU - Marjayandari, Lisa
AU - Istiqomah, Siti
AU - Dewi, Selva Rosyta
AU - Hanifah, Nabela Nur
AU - Sholichah, Mar'Atus
AU - Fatimah,
AU - Ni'Matuzahroh,
N1 - Publisher Copyright:
© 2018 Author(s).
PY - 2018/10/10
Y1 - 2018/10/10
N2 - Microbial existence is one of the standard indicators of room quality for stem cell culture laboratories by the Indonesian Health Ministry. During renovation of the Stem Cell Culture Laboratory in Surabaya, the laboratory technician designed a temporary laboratory for stem cell culture (SCC) on a research scale. The aim of this study was to examine sterility of the SCC processing room to determine whether it met the standard implemented by the Health Ministry of Indonesia. Microorganisms were isolated using direct plate, swap, and pour plate methods. Samples were collected from three points in the room. Equipment tested included CO2 incubator, biological safety cabinet, glass beaker, iodine cup, measuring pipettes, gloves, and Schott bottles. SCC media tested for microbes included adipose transport media, growth media, and media suspected of being contaminated by microbes. Descriptive analysis was based on microbial populations grown from each sample. Results showed that positive samples of bacterial infections included CO2 incubator, Biological Safety Cabinet, glass beakers, iodine cup, gloves, Schott bottle, and cell culture media suspected of microbial contamination. All samples were positive for infection by moulds and yeasts, except room, BSC, and growth media. This study indicated that the temporary laboratory did not meet stem cell culture room sterility standards set by the Health Ministry of Indonesia yet.
AB - Microbial existence is one of the standard indicators of room quality for stem cell culture laboratories by the Indonesian Health Ministry. During renovation of the Stem Cell Culture Laboratory in Surabaya, the laboratory technician designed a temporary laboratory for stem cell culture (SCC) on a research scale. The aim of this study was to examine sterility of the SCC processing room to determine whether it met the standard implemented by the Health Ministry of Indonesia. Microorganisms were isolated using direct plate, swap, and pour plate methods. Samples were collected from three points in the room. Equipment tested included CO2 incubator, biological safety cabinet, glass beaker, iodine cup, measuring pipettes, gloves, and Schott bottles. SCC media tested for microbes included adipose transport media, growth media, and media suspected of being contaminated by microbes. Descriptive analysis was based on microbial populations grown from each sample. Results showed that positive samples of bacterial infections included CO2 incubator, Biological Safety Cabinet, glass beakers, iodine cup, gloves, Schott bottle, and cell culture media suspected of microbial contamination. All samples were positive for infection by moulds and yeasts, except room, BSC, and growth media. This study indicated that the temporary laboratory did not meet stem cell culture room sterility standards set by the Health Ministry of Indonesia yet.
KW - microbial
KW - stem cell culture
KW - temporary laboratory
UR - http://www.scopus.com/inward/record.url?scp=85055480371&partnerID=8YFLogxK
U2 - 10.1063/1.5061881
DO - 10.1063/1.5061881
M3 - Conference contribution
AN - SCOPUS:85055480371
T3 - AIP Conference Proceedings
BT - 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University
A2 - Huyop, Fahrul Zaman Bin
A2 - Siswanto, Dian
A2 - Mastuti, Retno
A2 - Treesubsuntorn, Chairat
PB - American Institute of Physics Inc.
T2 - 9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018
Y2 - 7 March 2018 through 8 March 2018
ER -