TY - JOUR
T1 - Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction
AU - Effendi, Mustofa Helmi
AU - Hisyam, Mirza Atikah Madarina
AU - Hastutiek, Poedji
AU - Tyasningsih, Wiwiek
N1 - Funding Information:
The authors would like to thank the Ministry of Research, Technology and Higher Education of Indonesia for providing research funds with grant numbers; 893/UN3/2017 on the research title: PENANGGULANGAN ANTIBIOTICS RESISTANT BERSUMBER PANGAN ASAL HEWAN (PAH) MELALUI DETEKSI DINI DENGAN POLYMERASE CHAIN REACTION. This article is part of the research.
Publisher Copyright:
Copyright: Effendi, et al.
PY - 2019
Y1 - 2019
N2 - Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus.
AB - Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus.
KW - Coagulase gene
KW - Coagulase test
KW - Polymorphism
KW - Raw milk
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85062945369&partnerID=8YFLogxK
U2 - 10.14202/vetworld.2019.68-71
DO - 10.14202/vetworld.2019.68-71
M3 - Article
AN - SCOPUS:85062945369
SN - 0972-8988
VL - 12
SP - 68
EP - 71
JO - Veterinary World
JF - Veterinary World
IS - 1
ER -