TY - GEN
T1 - Detection of Candidatus Liberibacter asiaticus in the relatively new area of citrus plantation (Citrus spp.) in the absent of insect vector Diaphorina citri Kuw in Taro Village, Gianyar, Bali, Indonesia
AU - Wirawan, I. Gede Putu
AU - Dewi, Putu Alita
AU - Wijaya, I. Nyoman
AU - Adiartayasa, Wayan
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/23
Y1 - 2019/12/23
N2 - Six species of citrus plants visually observe at citrus plantations in Taro Village, Gianyar, Bali, Indonesia. The village is relatively new area for citrus that was previously reported free from citrus disease, citrus vein phloem degeneration (CVPD) or citrus greening or huanglongbing that caused by Candidatus Liberibacter asiaticus. The six citrus plants were Citrus nobilis, C. amblycarpa, C. reticulata, C. aurantifolia, C. limon and C. maxima. Six species of citrus plants found in the area which showed the CVPD like symptom. Samples from these six citrus species were then analyzed by using polymerase chain reaction (PCR) using 16S rDNA fragments as primers (O11 and O12c) to identify the bacteria, L. asiaticus. The result of this study showed that five of six citrus plants in Taro village were positively infected by L. asiaticus. While one species, C. limon, was negatively infected, the result suggested that C. limon may infected by other organisms or caused by other factors. In addition, we did not found any Diaphorina citri Kuw. in the citrus plantation. This result indicated that the citrus plants in Taro village infected by CVPD disease and the disease spread was by seedling which were made through occulation and brought from other area that has been infected by the disease.
AB - Six species of citrus plants visually observe at citrus plantations in Taro Village, Gianyar, Bali, Indonesia. The village is relatively new area for citrus that was previously reported free from citrus disease, citrus vein phloem degeneration (CVPD) or citrus greening or huanglongbing that caused by Candidatus Liberibacter asiaticus. The six citrus plants were Citrus nobilis, C. amblycarpa, C. reticulata, C. aurantifolia, C. limon and C. maxima. Six species of citrus plants found in the area which showed the CVPD like symptom. Samples from these six citrus species were then analyzed by using polymerase chain reaction (PCR) using 16S rDNA fragments as primers (O11 and O12c) to identify the bacteria, L. asiaticus. The result of this study showed that five of six citrus plants in Taro village were positively infected by L. asiaticus. While one species, C. limon, was negatively infected, the result suggested that C. limon may infected by other organisms or caused by other factors. In addition, we did not found any Diaphorina citri Kuw. in the citrus plantation. This result indicated that the citrus plants in Taro village infected by CVPD disease and the disease spread was by seedling which were made through occulation and brought from other area that has been infected by the disease.
UR - http://www.scopus.com/inward/record.url?scp=85077912140&partnerID=8YFLogxK
U2 - 10.1063/1.5141282
DO - 10.1063/1.5141282
M3 - Conference contribution
AN - SCOPUS:85077912140
T3 - AIP Conference Proceedings
BT - Proceedings of the 2nd International Conference on Bioscience, Biotechnology, and Biometrics 2019
A2 - Prasedya, Eka Sunarwidhi
A2 - Martyasari, Ni Wayan Riyani
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Bioscience, Biotechnology, and Biometrics 2019, ICBBB 2019
Y2 - 13 August 2019 through 14 August 2019
ER -