Detection mecA gene and Staphylococcus aureus resistance to several antibiotics isolated from cat ear swabs at a veterinary hospital located at Surabaya – Indonesia

Sergius Erikson Kaben, Mohammad Anam Al-Arif, Anwar Ma’ruf, Mustofa Helmi Effendi, Shendy Canadya Kurniawan, Katty Hendriana Priscilia Riwu, Daniah Ashri Afnani, Otto Sahat Martua Silaen, Saumi Kirey Millannia, Safira Ramadhani, Agus Widodo, Thoriq Ihza Farizqi, Aswin Rafif Khairullah

Research output: Contribution to journalArticlepeer-review

Abstract

Cats are susceptible to S. aureus, which mainly colonizes the nose and ears of these feline species. Otitis externa in cat ears is one of the illnesses produced by S. aureus in animals. Antibiotic therapy for affected animals is the conventional treatment for infections by S. aureus. Antibiotic use during prolonged treatment and given at the wrong doses can cause germs to become resistant. Given this context, research on S. aureus isolated from cat ears and tests for antibiotic resistance and the mecA gene is required. Samples of cat ears were obtained from the Amies media using a sterile cotton swab. Bacterial isolation was done on MSA media, and then the catalase and coagulase assays were used to identify the bacteria. S. aureus isolates were evaluated for sensitivity using disks of the antibiotics cefoxitin, tetracycline, erythromycin, gentamicin, and chloramphenicol connected to MHA media. All positive isolates of S. aureus underwent MRSA testing, and then the mecA gene was detected. The sample investigation revealed that 91% (91/100) were positive for S. aureus, and 3.30% (3/91) were confirmed to be multidrug-resistant (MDR) because they are resistant to 3–4 antibiotic classes. Out of the 12 MRSA isolates analyzed, the mecA gene was detected in one isolate. Inappropriate antibiotic use causes bacterial resistance in pets. Additionally, excessive antibiotic use in a population might develop acquired bacterial resistance to an antibiotic. Antibiotic use in animals must be assessed to administer medication and prevent the development of antibiotic resistance appropriately.

Original languageEnglish
Article numbere209027
JournalBrazilian Journal of Veterinary Research and Animal Science
Volume61
DOIs
Publication statusPublished - 12 Apr 2024

Keywords

  • Cat
  • Multi-drug resistance
  • Public health
  • Staphylococcus aureus

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