Hemoglobin is the protein molecule that binds oxygen in the blood. Hemoglobin level can be used as a benchmark of the human physiological condition, such as anemia, hypoxia, and postoperative bleeding. Generally, measurement of hemoglobin levels is performed by an invasive method that takes blood samples to be tested. This study offers a tele-monitoring system for measuring the hemoglobin level non-invasively, so it could reduce the possibility of infection or contamination to the body and can be monitored anywhere by using a website. This tele-monitoring device of non-invasive hemoglobin and oxygen saturation levels were designed by integrating the pulse oximetry sensor with signal conditioning circuit, Arduino Uno as a microcontroller, Ethernet module, an internet network, cloud server, and website. Pulse oximetry measured the oxygen saturation in the blood that indicate the vital state of human and became essential to determine the amount of hemoglobin level. The system performance in this study was tested on participants who had a good daily exercise and participants who never had daily activities to discover the correlation between hemoglobin levels with human physical activity and obtained 89% of accuracy. This system can be implemented as a personal device for monitoring the hemoglobin level and the oxygen saturation in daily used.