TY - JOUR
T1 - Densitometric determination of allylestrenol in tablets, and validation of the method
AU - Tampubolon, Hoshi Berniati
AU - Sumarlik, Endang
AU - Yuwono, Mochammad
AU - Indrayanto, Gunawan
PY - 2005
Y1 - 2005
N2 - A simple and rapid densitometric method has been developed for determination of allylestrenol in tablets. After extracting the samples with 96% ethanol, the solutions were spotted on pre-coated silica gel TLC plates, which were eluted with a mixture of n-hexane-ethyl acetate-dichloromethane (9.0:2.0:0.2, v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the allylestrenol spots at λ = 609 nm, after reaction with anisaldehyde-sulphuric acid reagent (5 min, 100°C). The TLC-densitometry method is selective, precise, and accurate, and can be used for routine analysis of tablets in pharmaceutical industry quality control laboratories.
AB - A simple and rapid densitometric method has been developed for determination of allylestrenol in tablets. After extracting the samples with 96% ethanol, the solutions were spotted on pre-coated silica gel TLC plates, which were eluted with a mixture of n-hexane-ethyl acetate-dichloromethane (9.0:2.0:0.2, v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the allylestrenol spots at λ = 609 nm, after reaction with anisaldehyde-sulphuric acid reagent (5 min, 100°C). The TLC-densitometry method is selective, precise, and accurate, and can be used for routine analysis of tablets in pharmaceutical industry quality control laboratories.
KW - Allylestrenol
KW - Densitometry
KW - Tablet
KW - TLC
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=12344278857&partnerID=8YFLogxK
U2 - 10.1081/JLC-200041332
DO - 10.1081/JLC-200041332
M3 - Article
AN - SCOPUS:12344278857
SN - 1082-6076
VL - 28
SP - 267
EP - 275
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 2
ER -