Densitometric determination of allylestrenol in tablets, and validation of the method

Hoshi Berniati Tampubolon, Endang Sumarlik, Mochammad Yuwono, Gunawan Indrayanto

Research output: Contribution to journalArticlepeer-review


A simple and rapid densitometric method has been developed for determination of allylestrenol in tablets. After extracting the samples with 96% ethanol, the solutions were spotted on pre-coated silica gel TLC plates, which were eluted with a mixture of n-hexane-ethyl acetate-dichloromethane (9.0:2.0:0.2, v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the allylestrenol spots at λ = 609 nm, after reaction with anisaldehyde-sulphuric acid reagent (5 min, 100°C). The TLC-densitometry method is selective, precise, and accurate, and can be used for routine analysis of tablets in pharmaceutical industry quality control laboratories.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalJournal of Liquid Chromatography and Related Technologies
Issue number2
Publication statusPublished - 2005


  • Allylestrenol
  • Densitometry
  • Tablet
  • TLC
  • Validation


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