TY - JOUR
T1 - Dengue virus serotype 4 is responsible for the outbreak of dengue in East Java City of Jember, Indonesia
AU - Aryati, Aryati
AU - Wrahatnala, Billy J.
AU - Yohan, Benediktus
AU - Fanny, May
AU - Hakim, Faradila K.N.
AU - Sunari, Eka Putri
AU - Zuroidah, Nelly
AU - Wardhani, Puspa
AU - Santoso, Marsha S.
AU - Husada, Dominicus
AU - Rohman, Ali
AU - Tarmizi, Siti Nadia
AU - Sievers, Justus T.O.
AU - Tedjo Sasmono, R.
N1 - Funding Information:
This study was funded by a research grant from the Universitas Airlangga (Grant number 965/UN3.14/LT/2019) to A.A.
Funding Information:
Funding: This study was funded 965/UN3.14/LT/2019) to A.A.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/9
Y1 - 2020/9
N2 - Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Strikingly, we observed the predominance of DENV-4, followed by DENV-3, DENV-1, and DENV-2. Genotype determination using Envelope gene sequence revealed the classification into Genotype I, Cosmopolitan Genotype, Genotype I, and Genotype II for DENV-1, -2, -3, and -4, respectively. The predominance of DENV-4 in Jember may be associated with a new wave of DENV infections and spread in a non-immune population lacking a herd-immunity to this particular serotype.
AB - Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Strikingly, we observed the predominance of DENV-4, followed by DENV-3, DENV-1, and DENV-2. Genotype determination using Envelope gene sequence revealed the classification into Genotype I, Cosmopolitan Genotype, Genotype I, and Genotype II for DENV-1, -2, -3, and -4, respectively. The predominance of DENV-4 in Jember may be associated with a new wave of DENV infections and spread in a non-immune population lacking a herd-immunity to this particular serotype.
KW - Dengue
KW - Indonesia
KW - Java
KW - Molecular epidemiology
KW - Serotype
UR - http://www.scopus.com/inward/record.url?scp=85089794807&partnerID=8YFLogxK
U2 - 10.3390/v12090913
DO - 10.3390/v12090913
M3 - Article
C2 - 32825262
AN - SCOPUS:85089794807
SN - 1999-4915
VL - 12
JO - Viruses
JF - Viruses
IS - 9
M1 - 913
ER -