Dengue virus infection-enhancing and neutralizing antibody balance in children of the Philippines and Indonesia

Atsushi Yamanaka, Yuko Tabuchi, Kris C. Mulyatno, Helen Susilowati, Eryk Hendrianto, Soegeng Soegijanto, Eiji Konishi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Dengue fever and dengue hemorrhagic fever are important diseases worldwide. Although antibody-dependent enhancement of infection has been proposed as a mechanism for increased disease severity, enhancing antibodies in endemic people have not been thoroughly investigated. Recently, we established a serological assay system to measure the balance of enhancing and neutralizing activities, which provides useful information for estimating in vivo antibody status. We measured the balance of these activities against four dengue virus (DENV) types in endemic populations, and analyzed the proportion of sera containing enhancing and neutralizing antibodies. Predominantly healthy Filipino children were used for analysis, although a population of Indonesian children was also investigated. In the Filipino population, the highest proportion of neutralizing activities was shown against DENV2, followed by DENV1. A greater proportion of sera exhibited enhancing rather than neutralizing antibodies against other virus types. Neutralizing activities were complement-dependent, while enhancing activities were complement-independent. The Indonesian population showed a similar dengue antibody status. Our results indicate that a relatively high proportion of endemic children possessed complement-independent enhancing antibodies against some DENV types.

Original languageEnglish
Pages (from-to)1152-1159
Number of pages8
JournalMicrobes and Infection
Issue number13
Publication statusPublished - Nov 2012


  • Antibody-dependent enhancement
  • Dengue
  • Epidemiology
  • Indonesia
  • Neutralization tests
  • Philippines


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