Implant placement in defective alveolar bone requires bone grafting and autogenous bone graft remains the gold standard bone material, however, it causes donor site morbidities. We explore the profile of newly developed demineralized freeze-dried bovine bone xenograft (DFDBBX). Objective: to assess profiles of DFDBBX granules. Enzymatic protein extraction of DFDBBX were performed and specific growth factors quantified with ELISA. For compatibility study rBM-MSCs was cultured in 2.5%, 5.0% and 10% DFDBBX medium for 72 hours followed by MTT assay. Human adipose-derived MSCs was cultured in conditioned medium of DFDBBX for 2, 7, and 14 days. Expression of RUNX2 and alkaline phosphatase (ALP) were examined by immunofluorescence assay. Data was analysed statistically with significance set at p-value < 0.05. PDGF, VEGF, and FGFα levels of DFDBBX were significantly higher, BMP2 and BMP4 levels were found comparable in both groups, whereas TGFβ1 significantly lower than FDBBX. Cell viability was comparable between DFDBBX and Deproteinized Bovine Bone Material (DBBM). Runx2 and ALP expression in DFDBBX is equivalent to osteogenic medium group and detected until 14 days. DFDBBX granules contain major proliferative and osteogenic growth factors, not cytotoxic and has osteoinductive properties equivalent to DBBM.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalJournal of International Dental and Medical Research
Issue number2
Publication statusPublished - 2021


  • Bone Graft
  • Demineralized Freeze-dried
  • Osteoblast
  • Osteoinduction
  • Xenograft


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