TY - JOUR
T1 - Demineralized Freeze-dried Bovine Bone Xenograft Granules as Alveolar Bone Substitutes
T2 - A Profile Study
AU - Barus, Liska
AU - Septianingtyas, Virna
AU - Febriadi, Pramudya Bagas
AU - Hendra, I. Made
AU - Annis, Arina Farmalabitta
AU - Satria, RM Haffiyan
AU - Wibisono, Natalia Ika
AU - Pratiwi, Ellen Satya
AU - Rasyida, Anindita Zahratur
AU - Mulyawan, Indra
AU - Rizqiawan, Andra
AU - Sumarta, Ni Putu Mira
AU - Danudiningrat, Coen Pramono
AU - Kamadjaja, David Buntoro
AU - Amir, Muhammad Subhan
N1 - Funding Information:
This work was supported by The Ministry of Research Technology and Higher Education, Republic of Indonesia, fiscal year 2020.
Publisher Copyright:
© 2021. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Implant placement in defective alveolar bone requires bone grafting and autogenous bone graft remains the gold standard bone material, however, it causes donor site morbidities. We explore the profile of newly developed demineralized freeze-dried bovine bone xenograft (DFDBBX). Objective: to assess profiles of DFDBBX granules. Enzymatic protein extraction of DFDBBX were performed and specific growth factors quantified with ELISA. For compatibility study rBM-MSCs was cultured in 2.5%, 5.0% and 10% DFDBBX medium for 72 hours followed by MTT assay. Human adipose-derived MSCs was cultured in conditioned medium of DFDBBX for 2, 7, and 14 days. Expression of RUNX2 and alkaline phosphatase (ALP) were examined by immunofluorescence assay. Data was analysed statistically with significance set at p-value < 0.05. PDGF, VEGF, and FGFα levels of DFDBBX were significantly higher, BMP2 and BMP4 levels were found comparable in both groups, whereas TGFβ1 significantly lower than FDBBX. Cell viability was comparable between DFDBBX and Deproteinized Bovine Bone Material (DBBM). Runx2 and ALP expression in DFDBBX is equivalent to osteogenic medium group and detected until 14 days. DFDBBX granules contain major proliferative and osteogenic growth factors, not cytotoxic and has osteoinductive properties equivalent to DBBM.
AB - Implant placement in defective alveolar bone requires bone grafting and autogenous bone graft remains the gold standard bone material, however, it causes donor site morbidities. We explore the profile of newly developed demineralized freeze-dried bovine bone xenograft (DFDBBX). Objective: to assess profiles of DFDBBX granules. Enzymatic protein extraction of DFDBBX were performed and specific growth factors quantified with ELISA. For compatibility study rBM-MSCs was cultured in 2.5%, 5.0% and 10% DFDBBX medium for 72 hours followed by MTT assay. Human adipose-derived MSCs was cultured in conditioned medium of DFDBBX for 2, 7, and 14 days. Expression of RUNX2 and alkaline phosphatase (ALP) were examined by immunofluorescence assay. Data was analysed statistically with significance set at p-value < 0.05. PDGF, VEGF, and FGFα levels of DFDBBX were significantly higher, BMP2 and BMP4 levels were found comparable in both groups, whereas TGFβ1 significantly lower than FDBBX. Cell viability was comparable between DFDBBX and Deproteinized Bovine Bone Material (DBBM). Runx2 and ALP expression in DFDBBX is equivalent to osteogenic medium group and detected until 14 days. DFDBBX granules contain major proliferative and osteogenic growth factors, not cytotoxic and has osteoinductive properties equivalent to DBBM.
KW - Bone Graft
KW - Demineralized Freeze-dried
KW - Osteoblast
KW - Osteoinduction
KW - Xenograft
UR - http://www.scopus.com/inward/record.url?scp=85110655363&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85110655363
SN - 1309-100X
VL - 14
SP - 623
EP - 628
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -