Demineralized Dentin Material Sponge (DDMS) Promotes RUNX2 expression in Osteoblastogenesis

In cases of severe bone loss, natural healing does not occur because the body’s natural regenerative capacity is exceeded. Therefore, there is a need for alternative materials that can support the healing process with good osteointegrative properties. Osteointegration is the relationship between a synthetic biomaterial implanted in the body and the biological response of the host tissue. Osteoblasts play a role in the process of bone remodeling and cooperate with osteoclasts to balance bone formation and resorption and remove mature bone tissue to maintain bone homeostasis. RUNX2 is a member of a small domain of transcription factors that are important for differentiation and proliferation during the transition of mesenchymal stem cells to osteoprogenitor cells during osteoblast differentiation. The demineralized dentin material sponge derived from bovine dentin contains 70% hydroxyapatite, 20% organic matrix, and 10% water, contains organic and inorganic components similar to dentin and human bone, which enhances the expression of RUNX2. It can be used as a bone substitute material. In the process of osteoblast formation. The aim of this study was to determine the potential ability of demineralized dentin sponges to promote RUNX2 expression in osteoblastogenesis.