TY - JOUR
T1 - Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds
AU - Vidarta, Ramadhan Pramudya
AU - Kamadjaja, David Buntoro
AU - Danudiningrat, Coen Pramono
AU - Amir, Muhammad Subhan
AU - Rizqiawan, Andra
AU - Yuliati, Anita
AU - Darwin, Darmawan
N1 - Publisher Copyright:
Copyright © 2025 Dental Journal (Majalah Kedokteran Gigi)
PY - 2025/3
Y1 - 2025/3
N2 - Background: Mandibular defects, often caused by trauma, tumors, infections, and congenital issues, are commonly treated with bone grafts. Tissue engineering plays a crucial role in bone reconstruction, with scaffolds such as deproteinized bovine bone matrix (DBBM), freeze-dried bovine bone (FDBB), and decellularized FDBB (Dc-FDBB) being studied for their efficacy. Decellularization reduces the antigenic potential of FDBB. These scaffolds are designed to degrade within the body. Purpose: To analyze the weight loss and degradation rates of FDBB and Dc-FDBB materials, using DBBM as a control. Methods: This in vitro experimental study, conducted over 2 months, employed a cross-sectional approach to analyze the weight loss and degradation rates of FDBB, Dc-FDBB, and DBBM scaffolds in a simulated body fluid (SBF) solution. Results: Under dynamic immersion conditions, DBBM exhibited the highest daily weight loss at 0.741% and a degradation rate of 0.466 mg/cm2/day, followed by Dc-FDBB at 0.568% and 0.418 mg/ cm2/day and FDBB at 0.525% and 0.385 mg/cm2/day. Under static immersion conditions, DBBM also showed the highest weight loss at 0.255%, with a degradation rate of 0.165 mg/cm2/day, followed by Dc-FDBB at 0.245% and 0.163 mg/cm2/day, and FDBB at 0.168% with a degradation rate of 0.126 mg/cm2/day. Significant differences were observed between scaffold groups (p = 0.000). Conclusion: DBBM, Dc-FDBB, and FDBB scaffolds meet the optimal requirements for tissue engineering materials based on their weight loss and degradation rates. DBBM demonstrated the highest values among the scaffolds analyzed.
AB - Background: Mandibular defects, often caused by trauma, tumors, infections, and congenital issues, are commonly treated with bone grafts. Tissue engineering plays a crucial role in bone reconstruction, with scaffolds such as deproteinized bovine bone matrix (DBBM), freeze-dried bovine bone (FDBB), and decellularized FDBB (Dc-FDBB) being studied for their efficacy. Decellularization reduces the antigenic potential of FDBB. These scaffolds are designed to degrade within the body. Purpose: To analyze the weight loss and degradation rates of FDBB and Dc-FDBB materials, using DBBM as a control. Methods: This in vitro experimental study, conducted over 2 months, employed a cross-sectional approach to analyze the weight loss and degradation rates of FDBB, Dc-FDBB, and DBBM scaffolds in a simulated body fluid (SBF) solution. Results: Under dynamic immersion conditions, DBBM exhibited the highest daily weight loss at 0.741% and a degradation rate of 0.466 mg/cm2/day, followed by Dc-FDBB at 0.568% and 0.418 mg/ cm2/day and FDBB at 0.525% and 0.385 mg/cm2/day. Under static immersion conditions, DBBM also showed the highest weight loss at 0.255%, with a degradation rate of 0.165 mg/cm2/day, followed by Dc-FDBB at 0.245% and 0.163 mg/cm2/day, and FDBB at 0.168% with a degradation rate of 0.126 mg/cm2/day. Significant differences were observed between scaffold groups (p = 0.000). Conclusion: DBBM, Dc-FDBB, and FDBB scaffolds meet the optimal requirements for tissue engineering materials based on their weight loss and degradation rates. DBBM demonstrated the highest values among the scaffolds analyzed.
KW - DBBM
KW - Dc-FDBB
KW - FDBB
KW - biomaterial
KW - maxillofacial defect
KW - scaffold
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85217632744&partnerID=8YFLogxK
U2 - 10.20473/j.djmkg.v58.i1.p23-29
DO - 10.20473/j.djmkg.v58.i1.p23-29
M3 - Article
AN - SCOPUS:85217632744
SN - 1978-3728
VL - 58
SP - 23
EP - 29
JO - Dental Journal
JF - Dental Journal
IS - 1
ER -