Cytotoxicity test of tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell (BMSC) of rats using MTT assay method

Hani Plumeriastuti, Agesti Veva Kalista, Budiastuti, Mustofa Helmi Effendi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The aim of this study was to identify the cytotoxicity effect of Tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell using MTT assay. The sample of this research using bone marrow mesenchymal stem cell of rats. The Tithonia diversifolia leaf extract divide into 4 concentration (0.5%, 0.25%, 0.125%, and 0.0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using the MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability, calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50analyzed by probit analysis. The extract of Tithonia diversifolia leaf with the concentration of 0.5%, 0.25%, and 0.125% found to kill cell up to 50% and only the concentration 0.0625 of Tithonia diversifolia leaf extract with the highest cell viability. The LC50value of Tithonia diversifolia leaf extract on bone marrow mesenchymal of rat is 0.167%.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalSystematic Reviews in Pharmacy
Volume11
Issue number9
DOIs
Publication statusPublished - 2020

Keywords

  • Cytotoxicity
  • LC
  • MTT assay
  • Mesenchymal bone marrow stem cell of rats
  • Tithonia diversifolia leaf extract

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