TY - JOUR
T1 - Cytotoxicity test of tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell (BMSC) of rats using MTT assay method
AU - Plumeriastuti, Hani
AU - Kalista, Agesti Veva
AU - Budiastuti,
AU - Effendi, Mustofa Helmi
N1 - Publisher Copyright:
© 2020 EManuscript Technologies. All rights reserved.
PY - 2020
Y1 - 2020
N2 - The aim of this study was to identify the cytotoxicity effect of Tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell using MTT assay. The sample of this research using bone marrow mesenchymal stem cell of rats. The Tithonia diversifolia leaf extract divide into 4 concentration (0.5%, 0.25%, 0.125%, and 0.0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using the MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability, calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50analyzed by probit analysis. The extract of Tithonia diversifolia leaf with the concentration of 0.5%, 0.25%, and 0.125% found to kill cell up to 50% and only the concentration 0.0625 of Tithonia diversifolia leaf extract with the highest cell viability. The LC50value of Tithonia diversifolia leaf extract on bone marrow mesenchymal of rat is 0.167%.
AB - The aim of this study was to identify the cytotoxicity effect of Tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell using MTT assay. The sample of this research using bone marrow mesenchymal stem cell of rats. The Tithonia diversifolia leaf extract divide into 4 concentration (0.5%, 0.25%, 0.125%, and 0.0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using the MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability, calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50analyzed by probit analysis. The extract of Tithonia diversifolia leaf with the concentration of 0.5%, 0.25%, and 0.125% found to kill cell up to 50% and only the concentration 0.0625 of Tithonia diversifolia leaf extract with the highest cell viability. The LC50value of Tithonia diversifolia leaf extract on bone marrow mesenchymal of rat is 0.167%.
KW - Cytotoxicity
KW - LC
KW - MTT assay
KW - Mesenchymal bone marrow stem cell of rats
KW - Tithonia diversifolia leaf extract
UR - http://www.scopus.com/inward/record.url?scp=85096751889&partnerID=8YFLogxK
U2 - 10.31838/srp.2020.9.145
DO - 10.31838/srp.2020.9.145
M3 - Article
AN - SCOPUS:85096751889
SN - 0975-8453
VL - 11
SP - 1008
EP - 1013
JO - Systematic Reviews in Pharmacy
JF - Systematic Reviews in Pharmacy
IS - 9
ER -