Cytotoxicity test of hydroxyapatite extract of human dental crown as a scaffold material

The jawbone is an important component because it functions as a supporting structure of the teeth, regulates mineral homeostasis and protects various organs in the oral cavity.The number of tooth extraction cases in the Oral and Maxillofacial Surgery Clinic of Dental and Oral Hospital, Faculty of Dental Medicine, UniversitasAirlangga average in 2019 every month is 800 teeth. This shows that there is potential to use wasted crowns, such as HA, which will be used as scaffold candidates. Cytotoxicity of HA dental crowns as scaffold candidates will be investigated.Using hidroxyapatite (HA) Powder Preparation and Cytotoxicity Test. The absorbance results for all concentration groups are above 50%, which means HA of dental crowns is not toxic according to CD₅₀, which states the material is said to be toxic if the number of living cells is below 50%. Tooth extraction waste that can be used as HA material is not toxic.