TY - JOUR
T1 - Cytotoxicity Test of Cacao Pod Extract (Theobroma Cacao. L) in Human Periodontal Ligament Fibroblast Cells (HPdLF) as Root Canal Irrigation Material
AU - Yuanita, Tamara
AU - Nawira,
AU - Prameswari, H. Amanda Diah
AU - Subiyanto, Ari
AU - Sukaton, null
AU - Bhardwaj, Anuj
N1 - Funding Information:
This study is based on portions of a dissertation submitted by the authors, under the direction of the corresponding author, to the Conservative Dentistry Department, Universitas Airlangga. The authors declare no potential conflicts of interest concerning for to the authorship and publication of this article
Publisher Copyright:
© 2022,Journal of International Dental and Medical Research. All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - One of the requirements for material as root canal irrigants is having low toxicity level since irrigation solution have the possibility of extruding into periapical tissue. Ligament periodontal cells are the major cells that play a role when reaction occur in periapical tissues due to endodontic materials. Fibroblast cells are also known as cells that first react when irrigation solution extruded to periradicular. Cacao pod peel extract, on the other hand, is known to have antioxidant, antibacterial, and anti-inflammatory characteristic so that it can be used as an alternative irrigation solution derived from natural ingredients. Nevertheless, every new material must go through biocompatibility test first before it is applied to humans and made into a patent product. This research aims to analyzed the toxicity of cacao pod peel extract (Theobroma Cacao L.) on human periodontal ligament fibroblast (HPdLF) cells. Primary cell cultures of periodontal ligament fibroblast were prepared from extracted premolar teeth for orthodontic treatment reason. They were exposed to 10 groups of concentrations of cacao pod peel extract range from 3000 μg/ml to 5,8593 μg/ml and tested for toxicity using MTT Assay, followed by ELISA Reader.
AB - One of the requirements for material as root canal irrigants is having low toxicity level since irrigation solution have the possibility of extruding into periapical tissue. Ligament periodontal cells are the major cells that play a role when reaction occur in periapical tissues due to endodontic materials. Fibroblast cells are also known as cells that first react when irrigation solution extruded to periradicular. Cacao pod peel extract, on the other hand, is known to have antioxidant, antibacterial, and anti-inflammatory characteristic so that it can be used as an alternative irrigation solution derived from natural ingredients. Nevertheless, every new material must go through biocompatibility test first before it is applied to humans and made into a patent product. This research aims to analyzed the toxicity of cacao pod peel extract (Theobroma Cacao L.) on human periodontal ligament fibroblast (HPdLF) cells. Primary cell cultures of periodontal ligament fibroblast were prepared from extracted premolar teeth for orthodontic treatment reason. They were exposed to 10 groups of concentrations of cacao pod peel extract range from 3000 μg/ml to 5,8593 μg/ml and tested for toxicity using MTT Assay, followed by ELISA Reader.
KW - Cacao pod (theobroma cacao. l)
KW - Cytotoxicity
KW - Human and medicine
KW - Human periodontal ligament fibroblast cells
KW - Lethal concentration
UR - http://www.scopus.com/inward/record.url?scp=85145863858&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85145863858
SN - 1309-100X
VL - 15
SP - 1472
EP - 1478
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -