Cytotoxicity evaluation of phytochemicals from stingless bee (Tetragonula biroi) propolis

Rico Ramadhan, Syafrizal, Irawan Wijaya Kusuma, Swandari Paramita, Yhiya Amen, Yong Ung Kim, Widya Fatriasari, Kuniyoshi Shimizu, Enos Tangke Arung

Research output: Contribution to journalArticlepeer-review


Three prenylated flavonoids (1–3) were isolated from Tetragonula biroi propolis. The structures of the isolated compounds were characterized by NMR, IR, and UV spectroscopic and mass spectrometric analyses. The cytotoxicity activity of the crude extracts, fractions and the isolated compounds were established against four cell lines such as Caco-2, HeLa, MCF-7, and OVK-18. Among the tested compounds, compound 1 showed cytotoxicity activity against MCF-7 cell lines, whereas compound 2 showed good activity against Caco-2 and OVK-18 cell lines with IC50 values of 14.73 and 14.44, respectively. Moreover, compound 3 exhibited strong activity against OVK-18 cell lines. These findings contribute to the phytochemical understanding of the T. biroi propolis, and their cytotoxicity effects for future pharmaceutical purposes.

Original languageEnglish
Article number107352
Publication statusPublished - Jan 2024


  • Anti-cancer
  • Cytotoxicity
  • Propolis
  • Tetragonula biroi


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