TY - JOUR
T1 - Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines
AU - Arung, Enos Tangke
AU - Ramadhan, Rico
AU - Khairunnisa, Binti
AU - Amen, Yhiya
AU - Matsumoto, Masako
AU - Nagata, Maki
AU - Kusuma, Irawan Wijaya
AU - Paramita, Swandari
AU - Sukemi,
AU - Yadi,
AU - Tandirogang, Nataniel
AU - Takemoto, Naomichi
AU - Syafrizal,
AU - Kim, Yong ung
AU - Shimizu, Kuniyoshi
N1 - Funding Information:
We were grateful for financially supported by the Ministry of Research, Technology and Higher Education, the Republic of Indonesia in the scheme Basic Research 2019 (Grant no. 198/UN17.41/KL/2019 for Enos Tangke Arung), Word Class Research (Grant no. 204/UN17.41/KL/2019 for Enos Tangke Arung), and Kyushu University Institute for Asian and Oceanian Studies (Q-AOS) for Kuniyoshi Shimizu. We also thanked Rendri Arista Avimaro (Samarinda) for providing the samples.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/12
Y1 - 2021/12
N2 - Our effort to find new material for anti cancer from natural resources leads us to focus on stingless bee products such as honey, bee pollen, and propolis. The products were from seven stingless bees named Homotrigona fimbriata, Heterotrigona itama, Heterotrigona bakeri, Tetragonula sarawakensis, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula laeviceps. The stingless bee products were evaluated for their cytotoxicity effect on MCF-7, HeLa and Caco-2 cancer cell lines. This is the first time to be reported that the honey, ethanol extracts of bee pollen and propolis of H. fimbriata displayed more potent cytotoxicity than other stingless bee products. By chromatography and biological activity-guided fractionation, ethanol extract of propolis from H. fimbriata was fractionated and isolated its active compound named mangiferonic acid. Mangiferonic acid showed a cytotoxicity effect with IC50 values 96.76 µM in MCF-7, >110.04 µM in HeLa, and > 110.04 µM in Caco-2, respectively. These results exhibited the potential of ethanol extracts from propolis of H. fimbriata to be further developed for drug and experiments to verify the function are essential.
AB - Our effort to find new material for anti cancer from natural resources leads us to focus on stingless bee products such as honey, bee pollen, and propolis. The products were from seven stingless bees named Homotrigona fimbriata, Heterotrigona itama, Heterotrigona bakeri, Tetragonula sarawakensis, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula laeviceps. The stingless bee products were evaluated for their cytotoxicity effect on MCF-7, HeLa and Caco-2 cancer cell lines. This is the first time to be reported that the honey, ethanol extracts of bee pollen and propolis of H. fimbriata displayed more potent cytotoxicity than other stingless bee products. By chromatography and biological activity-guided fractionation, ethanol extract of propolis from H. fimbriata was fractionated and isolated its active compound named mangiferonic acid. Mangiferonic acid showed a cytotoxicity effect with IC50 values 96.76 µM in MCF-7, >110.04 µM in HeLa, and > 110.04 µM in Caco-2, respectively. These results exhibited the potential of ethanol extracts from propolis of H. fimbriata to be further developed for drug and experiments to verify the function are essential.
KW - Bee pollen
KW - Cytotoxicity
KW - H. fimbriata
KW - Honey
KW - Propolis
KW - Stingless bees
UR - http://www.scopus.com/inward/record.url?scp=85113264580&partnerID=8YFLogxK
U2 - 10.1016/j.sjbs.2021.08.017
DO - 10.1016/j.sjbs.2021.08.017
M3 - Article
AN - SCOPUS:85113264580
SN - 1319-562X
VL - 28
SP - 7182
EP - 7189
JO - Saudi Journal of Biological Sciences
JF - Saudi Journal of Biological Sciences
IS - 12
ER -
