Cytoskeletal regulation on polycaprolactone/graphene porous scaffolds for bone tissue engineering

Hendrik Setia Budi; Silvia Anitasari; Yung Kang Shen; Shuntaro Yamada

doi:10.1038/s41598-024-80467-2

Cytoskeletal regulation on polycaprolactone/graphene porous scaffolds for bone tissue engineering

Hendrik Setia Budi, Silvia Anitasari, Yung Kang Shen, Shuntaro Yamada

Research output: Contribution to journal › Article › peer-review

Abstract

Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate the actin cytoskeleton, particularly filopodia and lamellipodia. Therefore, this study aims to investigate the effect of scaffolds properties on cell migration. The results showed that scaffolds toughness, strain, and strength played a key role in promoting cell movement by stimulating the dynamic formation of filopodia and lamellipodia. The test sample containing 3 wt% G significantly enhanced toughness, yield strength, and strain, leading to increase cell motility as well as enhanced development of longer filopodia and larger lamellipodia in MG-63 cells. These results provide valuable insights for optimizing scaffolds to promote bone tissue regeneration.

Original language	English
Article number	29062
Journal	Scientific Reports
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/s41598-024-80467-2
Publication status	Published - Dec 2024

Keywords

Cytoskeleton
Filopodia
Graphene
Lamellipodia
Polycaprolactone
Scaffolds

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10.1038/s41598-024-80467-2

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abstract = "Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate the actin cytoskeleton, particularly filopodia and lamellipodia. Therefore, this study aims to investigate the effect of scaffolds properties on cell migration. The results showed that scaffolds toughness, strain, and strength played a key role in promoting cell movement by stimulating the dynamic formation of filopodia and lamellipodia. The test sample containing 3 wt% G significantly enhanced toughness, yield strength, and strain, leading to increase cell motility as well as enhanced development of longer filopodia and larger lamellipodia in MG-63 cells. These results provide valuable insights for optimizing scaffolds to promote bone tissue regeneration.",

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AU - Budi, Hendrik Setia

AU - Anitasari, Silvia

AU - Shen, Yung Kang

AU - Yamada, Shuntaro

N1 - Publisher Copyright: © The Author(s) 2024.

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N2 - Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate the actin cytoskeleton, particularly filopodia and lamellipodia. Therefore, this study aims to investigate the effect of scaffolds properties on cell migration. The results showed that scaffolds toughness, strain, and strength played a key role in promoting cell movement by stimulating the dynamic formation of filopodia and lamellipodia. The test sample containing 3 wt% G significantly enhanced toughness, yield strength, and strain, leading to increase cell motility as well as enhanced development of longer filopodia and larger lamellipodia in MG-63 cells. These results provide valuable insights for optimizing scaffolds to promote bone tissue regeneration.

AB - Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate the actin cytoskeleton, particularly filopodia and lamellipodia. Therefore, this study aims to investigate the effect of scaffolds properties on cell migration. The results showed that scaffolds toughness, strain, and strength played a key role in promoting cell movement by stimulating the dynamic formation of filopodia and lamellipodia. The test sample containing 3 wt% G significantly enhanced toughness, yield strength, and strain, leading to increase cell motility as well as enhanced development of longer filopodia and larger lamellipodia in MG-63 cells. These results provide valuable insights for optimizing scaffolds to promote bone tissue regeneration.

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Cytoskeletal regulation on polycaprolactone/graphene porous scaffolds for bone tissue engineering

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Keywords

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