Cryoprotectant combination ethylene glycol and propanediol on mice blastocyst viability post vitrification

The best method to store embryo is vitrification using cryoprotectant, but so far, the viability of the embryo after warming process is decreasing. This objective of this research was to know the effect combination ethylene glycol and propanediol as intracellular cryoprotectant on mice blastocyst viability. The experimental animals were female mice strain Balb/C which was super ovulated using Pregnant Mare Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG). The oocyte collected from the ampulla of tuba fallopian. In Vitro Fertilization was done by placing the sperm and the oocyte in the same medium then cultured until became blastocyst. The blastocyst was classified into: T1: ethylene glycol 30%, T2: propanediol 30%, T3: ethylene glycol 20% and propanediol 10%, and T3: ethylene glycol 10% and propanediol 20%. Each group was vitrified for a week then warmed immediately. Examination of the viability of blastocyst was done using an inverted microscope after warming process. The statistical analysis showed that there were significant differences between single cryoprotectant and combination cryoprotectant group p<0.05. The best cryoprotectant combination was ethylene glycol 10% and propanediol 20%.