Crude methanol extract of brotowali leaves (Tinospora crispa) as biolarvacide against dengue vector aedes aegypti

This study aimed to determinate potential of crude methanol extract of brotowali leaves (MBL) as biolarvacide against Aeedes aegypti larva. Standard WHO bioassay test with slight modification were used. Third instar larva of Ae. aegypti were divided into control group and MBL treatment groups (concentrations of 25, 50, 100, 200, 500, 1000, 2000, 5000, 8000 and 10.000 ppm). Mortality was observed after 24 hours of exposure to MBL. MBL at concentration of 200, 500, 1000, 2000, 5000, 8000 and 10.000 ppm produced 6.67, 31.67, 40, 66.67, 98.33, 96.67, 98.33% larval mortality respectively. Larval mortality in the treatment groups were significantly different from control group (P<0.05). When the MBL concentrations increased, larval mortality was also raised. Probit analysis revealed that LC₅₀ and LC₉₀ of this study were 1205.092 and 4104.596 ppm. GC-MS test showed three dominant compounds in MBL, á-Methylphenylethylamine, Benzeneethanamine, and Methyl L-Alaninate. These compounds were classified as amphetamins, alkaloids, and amino acid. Phytochemistry test showed that brotowali leaves extract contained terpenoid. Terpenoid is the compound which usually found in bioinsecticide. Present study indicates that MBL has potential as safer biolarvacides against Aedes aegypti larva.