TY - JOUR
T1 - Contrast does not Affect Adipose Mesenchymal Stem Cell (AMSC) Proliferation
AU - Soekarno, G. R.
AU - Suryawan, I. G.R.
AU - Andrianto, A.
AU - Valentidenta, W. M.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2020/2/24
Y1 - 2020/2/24
N2 - In cardiology, stem cell therapy is currently being used. Adipose Mesenchymal Stem Cell (AMSC) is a cell culture expected to give a clinical improvement in patients. One of the methods to transfer the stem cell is cardiac catheterization assisted by contrast. There are no studies concerning the effect of contrast on proliferation or viability of stem cells. This study was designed to see the effect of contrast on the growth of AMSC culture in vitro. This research is an experimental post-test control group study. The samples were obtained from the thawing process at the Institute of Tropical Disease (ITD) Unair. The samples were cultured and checked immunohistochemically, divided into two groups, which are the treated group given iodixanol contrast and control group. The treated group was divided into se subgroups incubated 24 hours, which received 1mg/mL, 500g/ml, 250g/ml, 125g/ml, 62.5g/ml, 31.25g/ml and 15.625g/ml. AMSC proliferation was assessed by microtetrazolium (MTT) Cell Proliferation Assay and analyzed using T 2 free sample test and Analysis of Variance (ANOVA). MTT Cell Proliferation Assay showed no significant differences in AMSC proliferation on iodixanol contrast media compared to the control group. Iodixanol contrast does not affect AMSC proliferation.
AB - In cardiology, stem cell therapy is currently being used. Adipose Mesenchymal Stem Cell (AMSC) is a cell culture expected to give a clinical improvement in patients. One of the methods to transfer the stem cell is cardiac catheterization assisted by contrast. There are no studies concerning the effect of contrast on proliferation or viability of stem cells. This study was designed to see the effect of contrast on the growth of AMSC culture in vitro. This research is an experimental post-test control group study. The samples were obtained from the thawing process at the Institute of Tropical Disease (ITD) Unair. The samples were cultured and checked immunohistochemically, divided into two groups, which are the treated group given iodixanol contrast and control group. The treated group was divided into se subgroups incubated 24 hours, which received 1mg/mL, 500g/ml, 250g/ml, 125g/ml, 62.5g/ml, 31.25g/ml and 15.625g/ml. AMSC proliferation was assessed by microtetrazolium (MTT) Cell Proliferation Assay and analyzed using T 2 free sample test and Analysis of Variance (ANOVA). MTT Cell Proliferation Assay showed no significant differences in AMSC proliferation on iodixanol contrast media compared to the control group. Iodixanol contrast does not affect AMSC proliferation.
UR - http://www.scopus.com/inward/record.url?scp=85081177911&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/441/1/012174
DO - 10.1088/1755-1315/441/1/012174
M3 - Conference article
AN - SCOPUS:85081177911
SN - 1755-1307
VL - 441
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012174
T2 - 2nd International Conference on Fisheries and Marine Science, InCoFiMS 2019
Y2 - 26 September 2019
ER -