Continuous Subcultures Effect on Gynura procumbens Lour. (Merr.) Branch Number and Root Length

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Subculture is significant for maintaining the stock of roots to determine the proliferation of roots. Subcultures are needed to avoid an accumulation of toxic in the culture medium and container. In addition, there have been no reports regarding the maintenance of the adventitious root of Gynura procumbens through the subculture process. Therefore, we carried out this study is to determine the proliferation characters of adventitious root piece expiants by observing the total ofroot branches and measure the length ofroots during continued subcultures in a solid medium. The adventitious root of G. procumbens regenerated from leaf expiant in solid MS medium supplemented with 5 mg/L indole-3-butyric acid (IBA). The root pieces were subcultured with the same medium composition. Moreover, the subculture period was performed 5 t h time with a culture interval every 3 weeks. The result has shown that the subculture period affects a total of lateral root branches. The highest mean total of branches was achieved at the 4 t h subculture period (48.0±7.58), but not significant with the 1 s t subculture period (47.2±9.00). The length of the roots was affected by a total ofroot branches. The length of the roots was affected by a total of branches. The highest total roots branch on the 4 t h subculture has been dominated by high numbers root length with a score of 1 (31±2.04). On the other hand, the 1 s t subculture has dominated by high numbers root length with a score of 3 (26±0.83). The saturated concentration of I B A in medium and specific response from roots expiant tissues might cause different root proliferation during continued subcultures. In conclusion, continued subcultures of adventitious roots on certain periods can produce separate roots proliferation characters.

Original languageEnglish
Title of host publication8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
EditorsAnjar Tri Wibowo, M. Fariz Fadillah Mardianto, Riries Rulaningtyas, Satya Candra Wibawa Sakti, Muhammad Fauzul Imron, Rico Ramadhan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735442610
DOIs
Publication statusPublished - 25 Jan 2022
Event8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021 - Surabaya, Indonesia
Duration: 25 Aug 202126 Aug 2021

Publication series

NameAIP Conference Proceedings
Volume2554
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
Country/TerritoryIndonesia
CitySurabaya
Period25/08/2126/08/21

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