Compressive Strength and Porosity Size of Bovine-Gelatin-Chitosan Hydroxyapatite Scaffold

Hydroxyapatite can also be obtained from synthetic and natural materials, such as bovine tooth hydroxyapatite for bone graft. Gelatin is a type-I collagen which is considered to be the main organic component in bone. Chitosan has the same structure as glycosaminoglycans (GAGs), noncollagenous organic components of bone. This study aims to investigate the compressive strength and porosity of the HAGS-Gel-Kt scaffold with a ratio of 70:15:15 (w/w/w), Sigma-Aldrich Germany synthetic HA was used as comparison. HAGS:Gel:Kt scaffold was made with a ratio of 70:15:15, made with a weight of 1.75 gr HAGS powder, 0.375 gr gelatin and 0.375 gr chitosan. The HAGS-Gel-Kt scaffold used for pore size testing is a scaffold with a diameter of 5 mm and a height of 5 mm. The procedure for measuring the size of the porosity is carried out using scanning electron microsope (SEM). The compressive strength test was carried out using diameter of 8 mm and a height of 17 mm scaffold examined with universal testing instrument. HAGS-Gel-Kt cross-scaffold has higher size pore average than HAGS-Gel-Kt surface scaffold, HASyn-Gel-Kt surface scaffold, HASyn-Gel-Kt cross scaffold with significant different. HASyn-Gel- Kt has higher compressive strength than HAGS:Gel:Kt with significantly different (p<0.05). The compressive strength of the HAGS:Gel:Kt scaffold is equal to 0.221 MPa and the compressive strength of the HASyn:Gel:Kt scaffold is 0.370 MPa.