Composition variation of chitosan-gelatine scaffolds with glutaraldehyde cross linker for skin tissue engineering in burn wound cases

Burn wound is one type of trauma that has high morbidity and mortality. Skin tissue engineering for acceleraterepair of tissue damage in burn woundis needed The purpose of this research was to determine the best composition of the chitosan-gelatine from variation of chitosan-gelatine scaffolds with cross linker glutaraldehyde by the result of characterization of scaffolds. The making of scaffold was conducted by freeze drying techniques. The results of FTIR showed crosslinking between -NH2of chitosan and gelatine with C=O inglutaraldehyde. Tensile test was obtained at range 0,041-0,068 MPa value, all samples have tensile strength over tensile strength of the scaffold for skin application, which is 0,04 MPa. The morphology test resulted the diameter size of sample C and F, from 16,48-84,64 μm that met to pore size of scaffold for skin tissue engineering application. The cytotoxicity test demonstrated non-toxic scaffold with average of living cells above 90%. The degradation test obtained a value of 18,65%- 86,17% of the degraded weight during 21 days of observation. Based on the results of FTIR, tensile strength test, morphology test, cytotoxicity test and degradation test, chitosan-gelatine and glutaraldehyde scaffold is a potential candidate for skin tissue engineering in skin burn cases.