COMPARISON OF SERUM CYTOKINES IL-6, TNF-α, AND IL-10 AFTER SUBCUTANEOUS IMPLANTATION OF DECELLULARIZED AND NON DECELLULARIZED FREEZE DRIED BOVINE BONE SCAFFOLD (In vivo laboratory research on Rattus Novergicus)

Background: The xenogeneic scaffold such as FDBB is considered immunogenic due to the presence of xenogeneic cell residue. A decellularization process is added to FDBB to reduce the inflammatory response. Objectives: The objective of this study is to evaluate the efficacy of dc-FDBB in reducing the host inflammatory response after implantation, compared to DBBM as the gold standard scaffold with renowned biocompatibility. Method: Sixty Rattus Novergicus were divided into four groups, subcutaneously implanted FDBB, dc-FDBB, DBBM scaffolds and one group is sham-operated. On the 2nd, 7th and 14th postoperative days, a thoracotomy was performed and 1.5 ml of intra-cardiac blood was taken for ELISA test. Statistical tests were carried out by testing the normality of the data (Shapiro-Wilk) and homogeneity (Levene), then a comparative test was carried out with the Analysis of Variance (ANOVA) on data that was normally distributed and homogeneous, on data that was not normally distributed or not homogeneous, a comparison test with the Kruskall test Wallis, followed by the Post Hoc Tuckey HSD test (p<0.05). Results: Statistical analysis on days 2, 7 and 14 on levels of IL-6 (p = 0.530, p = 0.279, p = 0.790), TNF-α (p = 0.250, p = 0.009, p = 0.324), and IL-10 (p = 0.743, p = 0.349, p = 0.374 as a whole showed the result (p>0.05), there was no significant difference between all groups. Results of analysis of TNF-α day 7 (p=0.009) with (p > 0.05) showed that there was a difference, and continued with the post hoc test with the result p = 0.005, there was a difference between the controls compared to the dc-FDBB. Conclusion: There was no significant difference in serum levels of IL-6, TNF-α, and IL-10 after FDBB and dcFDBB implantation.