Comparison of nutrient-rich and limited media in the production of biosurfactant by Achromobacter xylosoxidans BP(1)5

Silvia Kurnia Sari, Ni’matuzahroh, Fatimah, Tri Nurhariyati, Nastiti Trikurniadewi, Ana Mariatul Khiftiyah, Achmad Zainal Abidin, Khudrotun Nisa Indriyasari

Research output: Contribution to journalArticlepeer-review


Aims: This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media. Methodology and results: This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m. Conclusion, significance and impact of study: The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium. The use of rice straw will contribute to the reduction of biosurfactant production costs and valorisation of agricultural waste

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalMalaysian Journal of Microbiology
Issue number2
Publication statusPublished - 2022


  • Achromobacter xylosoxidans
  • Agricultural and industrial wastes
  • Biosurfactant
  • Green product


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