TY - JOUR
T1 - Comparison of nutrient-rich and limited media in the production of biosurfactant by Achromobacter xylosoxidans BP(1)5
AU - Sari, Silvia Kurnia
AU - Ni’matuzahroh, null
AU - Fatimah,
AU - Nurhariyati, Tri
AU - Trikurniadewi, Nastiti
AU - Khiftiyah, Ana Mariatul
AU - Abidin, Achmad Zainal
AU - Indriyasari, Khudrotun Nisa
N1 - Funding Information:
This work was financially supported by “Direktorat Riset dan Pengabdian Masyarakat, Deputi Bidang Penguatan Riset dan Pengembangan Kementerian Riset dan Teknologi/ Badan Riset dan Inovasi Nasional” through “Penelitian Pendidikan Magister Menuju Doktor Sarjana Unggul” Universitas Airlangga scheme 1208/UN3.14/PT/2020.
Publisher Copyright:
© 2022. Malaysian Journal of Microbiology.All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - Aims: This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media. Methodology and results: This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m. Conclusion, significance and impact of study: The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium.
AB - Aims: This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media. Methodology and results: This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m. Conclusion, significance and impact of study: The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium.
KW - Achromobacter xylosoxidans
KW - Agricultural and industrial wastes
KW - Biosurfactant
KW - Green product
UR - http://www.scopus.com/inward/record.url?scp=85129719130&partnerID=8YFLogxK
U2 - 10.21161/mjm.211272
DO - 10.21161/mjm.211272
M3 - Article
AN - SCOPUS:85129719130
SN - 2231-7538
VL - 18
SP - 215
EP - 221
JO - Malaysian Journal of Microbiology
JF - Malaysian Journal of Microbiology
IS - 2
ER -