Comparison of α-Smooth Muscle Actin (α-SMA) and Collagen Type III Expression after Conjunctival Excision Between Platelet-Rich Fibrin (PRF) Membrane and Conjunctival Autograft Treatment: An Animal Model Experimental Study on Oryctolagus cuniculus

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Abstract

Purpose: To evaluate the impact of Platelet-Rich Fibrin (PRF) membrane and conjunctival autografts on α-Smooth Muscle Actin (α-SMA) and type III collagen expression following conjunctival excision in Oryctolagus cuniculus. Study Design: Experimental study with a randomized post-test only design. Place and Duration of Study: Airlangga University, Surabaya in November 2022. Methods: Twenty rabbit eyes were categorized into two groups: those sutured with PRF membrane and those sutured with conjunctival autografts after temporal conjunctival excision. The study assessed α-SMA and type III collagen expression through the Immunoreactive Score (IRS) method.Data analysis is conducted using appropriate statistical methods, such as the t-test or Mann-Whitney U test, to compare the expression of α-SMA and collagen type III between the two groups. Results: The data for each sample represented the average IRS value observed at 200x magnification. In the conjunctival autograft group, the mean IRS of α-SMA expression was 5.52 ± 0.84, significantly higher than the PRF membrane group (2.34 ± 0.34) with p < 0.05. Similarly, the conjunctival autograft group showed a higher mean IRS of type III collagen (3.87 ± 0.25) compared to the PRF membrane group (2.29 ± 0.31) (p < 0.05). Conclusion: Variations in α-SMA and type III collagen expression exist between the PRF membrane and conjunctival autograft groups in the rabbit corneal model, suggesting difference in their efficacy for conjunctival defect repair.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalPakistan Journal of Ophthalmology
Volume40
Issue number2
DOIs
Publication statusPublished - 1 Apr 2024

Keywords

  • autograft
  • conjunctiva
  • Platelet-Rich Fibrin
  • type III collagen
  • α-Smooth Muscle Actin

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