Comparing the Expression of OPG and RANKL in Wistar Rat Dental Sockets with Periodontitis Following the use of Nanohydroxyapatite and Microhydroxypatite

Nanohydroxyapatite is a nanometer-sized hydroxyapatite material that more closely resembles the form of natural hydroxyapatite found in bones and is absorbed more quickly by the body than microhydroxyapatite. In the human body, all the basic elements of bone are at the nano level. This is what researchers look at the expression of OPG and RANKL nanohydroxyapatite when compared to micro hydroxyapatite in the process of bone formation. This research used 60 rats divided into 12 groups such as negative control group, micro and nano group. Rats were induced with Porphyromonas gingivalis, and their teeth were extracted and grafted according to the groups. On the 3rd, 7th, 14th, and 21st day, the termination and preparations were made. Expression of OPG and RANKL was seen using IHC staining. There were significant differences in the expression of OPG and RANKL between the nanohydroxyapatite group and the control group and between the nano-hydroxyapatite and micro hydroxyapatite groups on days 3, 7, 14, 21. Conclusion: There is an increase in the expression of OPG and RANKL after the application of nano-hydroxyapatite compared to micro hydroxyapatite.