TY - JOUR
T1 - Comparative diagnostic value of anti-dengue IgG, anti-dengue IgM of two rapid tests in dengue virus infection
AU - Tanzilia, May Fanny
AU - Zuroidah, Nelly
AU - Ayu Eka Putri Sunari, I. Gusti Agung
AU - Wrahatnala, Billy Jordan
AU - Nisa, Faradila Khoirun
AU - Hakim,
AU - Rohman, Ali
AU - Wardhani, Puspa
AU - Husada, Dominicus
AU - Tarmizi, Siti Nadia
AU - Aryati,
N1 - Funding Information:
The authors would like to thank Universitas Airlangga for the funding of this research (Research grant number 965/UN3.14/LT/2019).
Publisher Copyright:
© 2020, Advanced Scientific Research. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Introduction: Diagnosis of Dengue Virus Infection (DVI) is based on combination of clinical manifestation, hematological, serological, and molecular examination. This study aims to compare the diagnostic value of anti-dengue IgG and anti-dengue IgM by two rapid tests with RT-PCR Dengue and ELISA as the gold standards. Methods: This was an observational cross-sectional study. Serum was collected from 80 febrile patients (3-7 day fever), who were suspected Dengue Virus Infection (DVI). Serum was examined by STANDARD Q-SD and SD BIOLINE as rapid tests and RT-PCR and or NS1 Ag Dengue (ELISA) and anti-dengue IgG/IgM ELISA as the gold standards. Results: The most common serotype identification was DENV 3. The positive results of NS1 Ag Dengue were common on day 3 of fever in primary infection and day 4 of fever in secondary infection. The antibody examination was dominated by positive anti-dengue IgM and positive anti-dengue IgG in 27 patients (54%) in secondary infection. Sensitivity anti-dengue IgG STANDARD Q was 68%, and SD BIOLINE was 87%. Specificity anti-dengue IgG STANDARD Q was 80%, and SD BIOLINE was 76.7%. Sensitivity IgM anti-dengue STANDARD Q was 44%, and SD BIOLINE was 48%. Specificity anti-dengue IgM of two rapid tests was 100%. Conclusion: The serological examination with rapid tests is not enough to establish a diagnosis of DVI, so the combination with NS1 Ag Dengue and RT-PCR examination is needed.
AB - Introduction: Diagnosis of Dengue Virus Infection (DVI) is based on combination of clinical manifestation, hematological, serological, and molecular examination. This study aims to compare the diagnostic value of anti-dengue IgG and anti-dengue IgM by two rapid tests with RT-PCR Dengue and ELISA as the gold standards. Methods: This was an observational cross-sectional study. Serum was collected from 80 febrile patients (3-7 day fever), who were suspected Dengue Virus Infection (DVI). Serum was examined by STANDARD Q-SD and SD BIOLINE as rapid tests and RT-PCR and or NS1 Ag Dengue (ELISA) and anti-dengue IgG/IgM ELISA as the gold standards. Results: The most common serotype identification was DENV 3. The positive results of NS1 Ag Dengue were common on day 3 of fever in primary infection and day 4 of fever in secondary infection. The antibody examination was dominated by positive anti-dengue IgM and positive anti-dengue IgG in 27 patients (54%) in secondary infection. Sensitivity anti-dengue IgG STANDARD Q was 68%, and SD BIOLINE was 87%. Specificity anti-dengue IgG STANDARD Q was 80%, and SD BIOLINE was 76.7%. Sensitivity IgM anti-dengue STANDARD Q was 44%, and SD BIOLINE was 48%. Specificity anti-dengue IgM of two rapid tests was 100%. Conclusion: The serological examination with rapid tests is not enough to establish a diagnosis of DVI, so the combination with NS1 Ag Dengue and RT-PCR examination is needed.
KW - Dengue virus
KW - Diagnosis
KW - ELISA
KW - Rapid test
UR - http://www.scopus.com/inward/record.url?scp=85090533269&partnerID=8YFLogxK
U2 - 10.31838/ijpr/2020.12.04.241
DO - 10.31838/ijpr/2020.12.04.241
M3 - Review article
AN - SCOPUS:85090533269
SN - 0975-2366
VL - 12
SP - 1657
EP - 1664
JO - International Journal of Pharmaceutical Research
JF - International Journal of Pharmaceutical Research
IS - 4
ER -
