Comparative diagnostic value of anti-dengue IgG, anti-dengue IgM of two rapid tests in dengue virus infection

May Fanny Tanzilia, Nelly Zuroidah, I. Gusti Agung Ayu Eka Putri Sunari, Billy Jordan Wrahatnala, Faradila Khoirun Nisa, Hakim, Ali Rohman, Puspa Wardhani, Dominicus Husada, Siti Nadia Tarmizi, Aryati

Research output: Contribution to journalReview articlepeer-review


Introduction: Diagnosis of Dengue Virus Infection (DVI) is based on combination of clinical manifestation, hematological, serological, and molecular examination. This study aims to compare the diagnostic value of anti-dengue IgG and anti-dengue IgM by two rapid tests with RT-PCR Dengue and ELISA as the gold standards. Methods: This was an observational cross-sectional study. Serum was collected from 80 febrile patients (3-7 day fever), who were suspected Dengue Virus Infection (DVI). Serum was examined by STANDARD Q-SD and SD BIOLINE as rapid tests and RT-PCR and or NS1 Ag Dengue (ELISA) and anti-dengue IgG/IgM ELISA as the gold standards. Results: The most common serotype identification was DENV 3. The positive results of NS1 Ag Dengue were common on day 3 of fever in primary infection and day 4 of fever in secondary infection. The antibody examination was dominated by positive anti-dengue IgM and positive anti-dengue IgG in 27 patients (54%) in secondary infection. Sensitivity anti-dengue IgG STANDARD Q was 68%, and SD BIOLINE was 87%. Specificity anti-dengue IgG STANDARD Q was 80%, and SD BIOLINE was 76.7%. Sensitivity IgM anti-dengue STANDARD Q was 44%, and SD BIOLINE was 48%. Specificity anti-dengue IgM of two rapid tests was 100%. Conclusion: The serological examination with rapid tests is not enough to establish a diagnosis of DVI, so the combination with NS1 Ag Dengue and RT-PCR examination is needed.

Original languageEnglish
Pages (from-to)1657-1664
Number of pages8
JournalInternational Journal of Pharmaceutical Research
Issue number4
Publication statusPublished - 1 Oct 2020


  • Dengue virus
  • Diagnosis
  • Rapid test


Dive into the research topics of 'Comparative diagnostic value of anti-dengue IgG, anti-dengue IgM of two rapid tests in dengue virus infection'. Together they form a unique fingerprint.

Cite this