Combination of papain enzyme and phytase enzyme in commercial feed and the protein and energy retention of tilapia Oreochromis niloticus

The nile tilapia (Oreochromis niloticus), is one of the important economic commodities in the fishery sector. In the process of the cultivation of the Nile Tilapia, there are many problems faced by cultivators; one of them is the expensive cost of the feed. An alternative to overcome this problem is by adding papain and fitase enzymes. This study aims to determine the effect and optimum dose of a combination of papain and fitase enzymes on the protein and energy retention of Nile Tilapia. This study used a Completely Randomized Design (RAL) with 4 treatments and 5 replications. The treatments used were P0 (control), P1 (papain 1% and fitase 0.05%), P2 (papain 3% and 0.1% fitase enzyme) and P3 (papain 5% and fitase 0.15%). The data was analyzed using ANOVA and continued with Duncan's multiple range test. The results of this study showed that the combination of papain and fitase enzymes in commercial feed affected the protein retention but did not affect the energy retention. A dose of 3% enzyme papain and 0.1% fitase enzyme (P2) was the optimum dose needed to increase the protein retention of Nile Tilapia fish.