Combination of dental follicle stem cells and nanocomposite fibrous scaffold for regeneration of post operative bone defect Ameloblastoma

Ameloblastoma is a benign odontogenic tumor in the jaw bone and can become malignant. Ameloblastoma covers 9-11% of all cases of odontogenic tumors present. The incidence of malignant ameloblastoma reaches 1.79 per 10 million people per year. Generally, as much as 80% of cases of ameloblastoma occur in the mandibular region with a posterior predilection. Ameloblastoma lesions are very progressive, invasive, and infiltrate the bone in the medullary spaces and cortical bone. In general, patients with ameloblastoma perform operative measures such as enucleation and curettage in the bone, even marginal or segmental resection can also be performed. However, after the operative procedure it causes a defect in the jaw bone. This condition, if not done regeneratively can cause effects such as speech disorders, masticatory disorders and deformity in the jawbone. The combination of dental follicle stem cells is planted in a nanocomposite fibrous scaffold to optimize the regeneration of osteogenic cells. Dental follicle stem cells and Nanocomposite fibrous scaffold have the ability as angiogenesis and osteoinductive. To analyze the potential combination of dental follicle stem cells and Nanocomposite fibrous scaffold to regenerate postoperative ameloblastoma bone defects. The use of dental follicle stem cell has the osteoinductive ability by increasing the expression of RunX2, Coll1, and ALP markers which play a role in inducing osteoblast formation. The use of Nanocomposite fibrous scaffold can release VEGF, FGF2, and BMP2 which are useful in stimulating vascularization and bone regeneration. The combination of dental follicle stem cells and nanocomposite fibrous scaffold can increase proliferation, differentiation and osteogenic stimulation of stem cells. The combination of dental follicle stem cells and nanocomposite fibrous scaffold has the potential to regenerate postoperative ameloblastoma bone defects.