The target of healing periodontal defects is the regeneration of periodontal tissue damaged by periodontal disease. It is known that type 1 collagen organic material as a bone graft material which has the advantage of being a bone graft material derived from animal sources (xenograft) capable of regenerating periodontal tissue. The purpose of this article is to illustrate how differences in collagen extracted by chemical and enzymatic hydrolysis when used for bone graft material in the regenerative therapy of periodontal tissue. The data source of this article was obtained from limited literature sourced from PubMed, ResearchGate and Google Scholar. No filter was applied to limit retrieval based on the type of study. All information discussed in this article was collected from international scientific multidisciplinary journals between 2007 and 2018 that discussed the process of collagen extraction and its benefits that have been studied. Collagen hydrolysis from the combination of acids, especially acetic acid and enzyme pepsin in the enzymatic hydrolysis process produce collagen with a high yield value and less of waste. Enzymatic hydrolysis also gives specific collagen extraction results, especially type I collagen which has a good denaturation temperature to be used as graft material in regenerative periodontal treatment.