Cloning, purification, and characterization of recombinant endo- β-1,4-D-xylanase of Bacillus sp. From soil termite abdomen

Eka Safitri, Hanifah, Previta, Sudarko, Ni Nyoman Tri Puspaningsih, Anak Agung Istri Ratnadewi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A novel endo-β-1,4-D-xylanase (xynBT) was identified from Bacillus sp. in soil termite abdomen and successfully cloned in Escherichia coli TOP10 and expressed in Escherichia coli BL21 (DE3) via pET-30a (+) as an expression vector. The full length gene consist of 801 bp ORF encoding a 267 amino acid polypeptide. The deduced amino acid sequence of xynBT displayed homology with glycoside hydrolase (GH) family 11 xylanase. Recombinant XynBT (r-XynBT), which was purified, showed an optimal pH and temperature of 5.5 and 40 °C, respectively. This enzyme was purified by the Immobilized Metal Affinity Chromatography (IMAC) method and has a molecular mass of 30 kDa, which was observed via sodium dodecyl polyacrylamide sodium electrophoresis (SDS-PAGE). Purified r-XynBT was the most stable at pH 5 for up to 120 min pre-incubation time and had a residual activity of 83%. Purified r-XynBT was also stable between 30 and 40 °C for 80 min of pre-incubation and had a residual activity of more than 50%. The presence of metal cations K+ and Na+ on r-XynBT increased its activity, while metal cations Mg2+, Cu2+, Zn2+, and Fe3+ were inhibitors.

Original languageEnglish
Article number101877
JournalBiocatalysis and Agricultural Biotechnology
Volume31
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Soil termite abdomen
  • Xylanase
  • purification

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