Clitoria Ternatea Ethanol Extract Antibacterial and Anti-Inflammatory Ability Towards Peri-Implantitis Biomarkers: An in Vitro and in Silico Study

Nastiti Faradilla Ramadhani, Real Akbar Aucky Sanjaya, Ratri Maya Sitalaksmi, Muhammad Dimas Aditya Ari, Alexander Patera Nugraha, Shafira Ninditya Irsan, Viol Dhea Kharisma, Tengku Natasha Eleena binti Tengku Ahmad Noor

Research output: Contribution to journalArticlepeer-review

Abstract

Clitoria ternatea may possess antimicrobial, antioxidant, and anti-inflammatory activities that are expected to provide benefits for dental peri-implantitis treatment. Objective to investigate C. ternatea ethanolic extract (CTEE) antibacterial activity toward periimplantitis bacteria in vitro and C. ternatea’s anthocyanin anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE was examined with phytochemical tests, antioxidant activity tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests to analyze antibacterial activity toward dental peri-implantitis bacteria such as Prophyromonas gingivalis (Pi), Aggregatibacter actinomycetemcomitans (Aa), Provotella intermedia (Pi), and Fusobacteria nucleatum (Fn). Molecular docking of CEET’s anthocyanin to tumor necrosis factor-α (TNF-α), nuclear factor kappa beta (NFkB), receptor activator nuclear kappa (RANK) and its ligand (RANKL), interleukin-6 (IL-6), osteoprotegrin (OPG), osteocalcin, nuclear factor associated T-cells 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, heat shock protein-10 (HSP-10) and -70 (HSP-70) was performed. The Shapiro-Wilk test, Levene’s test, and one-way analysis of variance (ANOVA) test continued with a post-hoc test (p<0.50) to analyze the data. CTEE contains active compounds including flavonoids, saponins, quinones, alkaloids, tannins, terpenoids, and steroids. CTEE has lower antioxidant activity than vitamin C. CTEE at 50% concentration has antibacterial activity that inhibits Aa, Pg, Pi, and Fn growth. Anthocyanins of CTEE show anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE has antibacterial activity towards Aa, Pg, Pi, and Fn at 50% concentration and potential antioxidant ability in vitro. CTEE’s anthocyanin has anti-inflammatory, antimicrobial, and antioxidant activity in silico.

Original languageEnglish
Pages (from-to)1038-1049
Number of pages12
JournalJournal of International Dental and Medical Research
Volume16
Issue number3
Publication statusPublished - 2023

Keywords

  • Clitoria ternatea
  • Communicable disease
  • Dentistry
  • Medicine
  • Peri-implantitis

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