TY - JOUR
T1 - Clitoria Ternatea Ethanol Extract Antibacterial and Anti-Inflammatory Ability Towards Peri-Implantitis Biomarkers
T2 - An in Vitro and in Silico Study
AU - Ramadhani, Nastiti Faradilla
AU - Sanjaya, Real Akbar Aucky
AU - Sitalaksmi, Ratri Maya
AU - Ari, Muhammad Dimas Aditya
AU - Nugraha, Alexander Patera
AU - Irsan, Shafira Ninditya
AU - Kharisma, Viol Dhea
AU - Noor, Tengku Natasha Eleena binti Tengku Ahmad
N1 - Publisher Copyright:
© (2023), (University of Dicle). All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Clitoria ternatea may possess antimicrobial, antioxidant, and anti-inflammatory activities that are expected to provide benefits for dental peri-implantitis treatment. Objective to investigate C. ternatea ethanolic extract (CTEE) antibacterial activity toward periimplantitis bacteria in vitro and C. ternatea’s anthocyanin anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE was examined with phytochemical tests, antioxidant activity tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests to analyze antibacterial activity toward dental peri-implantitis bacteria such as Prophyromonas gingivalis (Pi), Aggregatibacter actinomycetemcomitans (Aa), Provotella intermedia (Pi), and Fusobacteria nucleatum (Fn). Molecular docking of CEET’s anthocyanin to tumor necrosis factor-α (TNF-α), nuclear factor kappa beta (NFkB), receptor activator nuclear kappa (RANK) and its ligand (RANKL), interleukin-6 (IL-6), osteoprotegrin (OPG), osteocalcin, nuclear factor associated T-cells 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, heat shock protein-10 (HSP-10) and -70 (HSP-70) was performed. The Shapiro-Wilk test, Levene’s test, and one-way analysis of variance (ANOVA) test continued with a post-hoc test (p<0.50) to analyze the data. CTEE contains active compounds including flavonoids, saponins, quinones, alkaloids, tannins, terpenoids, and steroids. CTEE has lower antioxidant activity than vitamin C. CTEE at 50% concentration has antibacterial activity that inhibits Aa, Pg, Pi, and Fn growth. Anthocyanins of CTEE show anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE has antibacterial activity towards Aa, Pg, Pi, and Fn at 50% concentration and potential antioxidant ability in vitro. CTEE’s anthocyanin has anti-inflammatory, antimicrobial, and antioxidant activity in silico.
AB - Clitoria ternatea may possess antimicrobial, antioxidant, and anti-inflammatory activities that are expected to provide benefits for dental peri-implantitis treatment. Objective to investigate C. ternatea ethanolic extract (CTEE) antibacterial activity toward periimplantitis bacteria in vitro and C. ternatea’s anthocyanin anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE was examined with phytochemical tests, antioxidant activity tests, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests to analyze antibacterial activity toward dental peri-implantitis bacteria such as Prophyromonas gingivalis (Pi), Aggregatibacter actinomycetemcomitans (Aa), Provotella intermedia (Pi), and Fusobacteria nucleatum (Fn). Molecular docking of CEET’s anthocyanin to tumor necrosis factor-α (TNF-α), nuclear factor kappa beta (NFkB), receptor activator nuclear kappa (RANK) and its ligand (RANKL), interleukin-6 (IL-6), osteoprotegrin (OPG), osteocalcin, nuclear factor associated T-cells 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, heat shock protein-10 (HSP-10) and -70 (HSP-70) was performed. The Shapiro-Wilk test, Levene’s test, and one-way analysis of variance (ANOVA) test continued with a post-hoc test (p<0.50) to analyze the data. CTEE contains active compounds including flavonoids, saponins, quinones, alkaloids, tannins, terpenoids, and steroids. CTEE has lower antioxidant activity than vitamin C. CTEE at 50% concentration has antibacterial activity that inhibits Aa, Pg, Pi, and Fn growth. Anthocyanins of CTEE show anti-inflammatory, antimicrobial, and antioxidant activity in silico. CTEE has antibacterial activity towards Aa, Pg, Pi, and Fn at 50% concentration and potential antioxidant ability in vitro. CTEE’s anthocyanin has anti-inflammatory, antimicrobial, and antioxidant activity in silico.
KW - Clitoria ternatea
KW - Communicable disease
KW - Dentistry
KW - Medicine
KW - Peri-implantitis
UR - http://www.scopus.com/inward/record.url?scp=85173508181&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85173508181
SN - 1309-100X
VL - 16
SP - 1038
EP - 1049
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -
