Introduction: There are various methods in cholesterol analysis, for example, amperometric, enzymatic, and electrochemistry. Those methods are not expressing the suitable methods for cholesterol analysis because of the complicated and long procedure. Methods: This experiment is developing cholesterol analysis methods using enzyme immobilization techniques. The enzyme, cholesterol oxidase, immobilized on polyaniline surface conducted chitosan and formed a biosensor. Polyaniline matrix bound to chitosan in a covalent bond, a strong bonding that acts as an enzyme's trap. Results: The optimal biosensor has 650 nm maximum wavelength and optimum pH at 7. Immobilization process occurs in 24 hours. The biosensor has 8 minutes response time and can be used one time during 2 weeks active time. The biosensor responses increase linearly at range 0.1 mg/mL and 2.5 mg/mL. The sensitivity at 0.304 mg/ml and accurate value at 89.72%. The detection limit and quantity limit are at 0.00521 mg/mL and 0.0197 mg/mL. Conclusion: The result showed that this method can be used as an eco-friendly material for a biosensor. This method can be developed by using the suitable crosslinker for enzyme-surface to enhance biosensor performance.
|Number of pages||6|
|Journal||Malaysian Journal of Medicine and Health Sciences|
|Publication status||Published - 2019|
- Cholesterol oxidase