Characterization of ultraviolet B light emitting diodes (UVB LED) irradiation device for Wistar rats as an experimental animal model

Background: Light emitting diodes (LED) lamps are used widely in daily because of their many benefits, including their low cost, extended lifespan, low energy consumption, and low environmental impact. The use of LEDs, especially ultraviolet B LED (UVB LEDs) is quite extensive and has been developed for the treatment of skin diseases, irradiating pet reptiles, and conducting research on experimental animals. The use of light for therapy must be done carefully so that unwanted side effects do not occur because light also harms tissue. The purpose of this characterization is to determine the stability of temperature and irradiation of the device we make and to determine the duration of light exposure based on the characterization. Methods: The irradiation device use UVB LEDs with a wavelength of 305-310 nm. The characterization carried out included temperature stability and irradiation stability, with observations every 30 minutes for 10 hours and no replication. Statistical analysis using the Kolmogorov-Smirnov (Asymptotic Approximation) testing technique. The test criteria state that if the probability value > level of significance (alpha (α) = 5%). Results: The characterization results from 10 hours of observation with sampling data taken every 30 minutes showed stable LED temperature and room temperature while unstable cage temperature and irradiance. Conclusion: This device can be considered to use in Wistar rat’ experimental studies using UVB and perhaps developed further. The duration of exposure can be adjusted according to the distance of the object and the dose required.