TY - JOUR
T1 - Characterization of Fungal Laccase Isolated from oil palm empty fruit bunches (OPEFB) and Its Degradation from The Agriculture Waste
AU - Fithri, Lailatul
AU - Puspaningsih, Ni Nyoman Tri
AU - Asmarani, One
AU - Ni'matuzahroh,
AU - Fitrah Dewi, Gytha Deasy
AU - Arizandy, Radita Yuniar
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/8
Y1 - 2020/8
N2 - In recent years, many studies have been developed to overcome the abundance of lignocellulosic waste. Laccase is a metalloenzyme that can degrade lignin. This enzyme has useful properties that make it applicable for the agricultural sector in Indonesia. The present study is designed to isolate and determine the activity of laccase found in the fungi of oil palm empty fruit bunch (OPEFB), and the fungal laccase was precipitated and partially purified by using ammonium sulfate. The optimum temperature of fungal laccase was 40 °C for fungus lacc-B and 50 °C for fungus lacc-A, C, and D. The optimum pH was obtained 5.0 for fungus lacc-A and D, pH 6.0 for fungus lacc-C and pH 7.0 for fungus lacc-B. Furthermore, the activity of crude extract were 1.4 U.mL−1, 1.5 U.mL−1, 1.3 U.mL−1, and 1.3 U.mL−1 for fungus lacc-A, B, C, and D. Purification levels of fungus lacc-A, B, C, and D increased 1.7, 1.6, 1.9, and 2.5 times, respectively after ammonium sulfate precipitation and dialysis. Fungus lacc-D has the highest level of laccase activity than other fungi. Physical analysis of the agricultural waste after fungus lacc-D addition indicated that the surface profile is damaged, hollow, and broken. The delignification from corn cob and rice straw was observed with scanning electron microscope (SEM) which showed the presence of morphological changes in the lignocellulose waste sample.
AB - In recent years, many studies have been developed to overcome the abundance of lignocellulosic waste. Laccase is a metalloenzyme that can degrade lignin. This enzyme has useful properties that make it applicable for the agricultural sector in Indonesia. The present study is designed to isolate and determine the activity of laccase found in the fungi of oil palm empty fruit bunch (OPEFB), and the fungal laccase was precipitated and partially purified by using ammonium sulfate. The optimum temperature of fungal laccase was 40 °C for fungus lacc-B and 50 °C for fungus lacc-A, C, and D. The optimum pH was obtained 5.0 for fungus lacc-A and D, pH 6.0 for fungus lacc-C and pH 7.0 for fungus lacc-B. Furthermore, the activity of crude extract were 1.4 U.mL−1, 1.5 U.mL−1, 1.3 U.mL−1, and 1.3 U.mL−1 for fungus lacc-A, B, C, and D. Purification levels of fungus lacc-A, B, C, and D increased 1.7, 1.6, 1.9, and 2.5 times, respectively after ammonium sulfate precipitation and dialysis. Fungus lacc-D has the highest level of laccase activity than other fungi. Physical analysis of the agricultural waste after fungus lacc-D addition indicated that the surface profile is damaged, hollow, and broken. The delignification from corn cob and rice straw was observed with scanning electron microscope (SEM) which showed the presence of morphological changes in the lignocellulose waste sample.
KW - Fungi
KW - Laccase
KW - Lignin
KW - Scanning electron microscope
UR - http://www.scopus.com/inward/record.url?scp=85086595111&partnerID=8YFLogxK
U2 - 10.1016/j.bcab.2020.101676
DO - 10.1016/j.bcab.2020.101676
M3 - Article
AN - SCOPUS:85086595111
SN - 1878-8181
VL - 27
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
M1 - 101676
ER -