TY - JOUR
T1 - Characterization of cellulolytic bacteria as candidate probiotic for animal
AU - BernyYulianto, Andreas
AU - WidyaParamitaLokapirnasari,
AU - RifkyNajwan,
AU - Huda, Khoirul
AU - CipkaPramudaWardhani, Hana
AU - Rahman, Nabil Fariz Noor
N1 - Publisher Copyright:
© 2019 Indian Veterinary Assocaition. All rights reserved.
PY - 2019
Y1 - 2019
N2 - This study was aimed to identify cellulolytic isolates from local bovine rumen. Cellulolytic activities of that isolate were evaluated to determine the activities of endoglucanase, exoglucanase and β-glucosidase. Genotypic testing was conducted by analysing 16S rDNA and biochemical identification of VITEK 2 microbial identification system version: 05.01 (BioMérieux). DNA of sample bacteria was isolated and further amplified in vitro with the PCR method. The nucleotide sequence of 16S rDNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. Based on the result, the cellulolytic isolate was identified as Enterobacter cloacae.
AB - This study was aimed to identify cellulolytic isolates from local bovine rumen. Cellulolytic activities of that isolate were evaluated to determine the activities of endoglucanase, exoglucanase and β-glucosidase. Genotypic testing was conducted by analysing 16S rDNA and biochemical identification of VITEK 2 microbial identification system version: 05.01 (BioMérieux). DNA of sample bacteria was isolated and further amplified in vitro with the PCR method. The nucleotide sequence of 16S rDNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. Based on the result, the cellulolytic isolate was identified as Enterobacter cloacae.
KW - 16S rDNA
KW - Biochemical test
KW - Cellulolytic enzyme activity
UR - http://www.scopus.com/inward/record.url?scp=85071841155&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85071841155
SN - 0019-6479
VL - 96
SP - 29
EP - 31
JO - Indian Veterinary Journal
JF - Indian Veterinary Journal
IS - 8
ER -