Characterization of Bovine Sponge Amnion (BSA) by a Novel Process for Dental Treatment

One of dental treatments in dentistry is tooth extraction. This treatment will cause injury after extraction and biomaterial is needed to accelerate wound healing. Bovine amniotic membrane is a biomaterial with collagen content that can accelerate wound healing. However, in the process of application, the membrane will tear easily and is difficult to apply. The purpose of this study was to fabricate the bovine amniotic membrane into bovine sponge amnion (BSA) and characterize it. BSA was derived from bovine amniotic membrane with the addition of gelatin which then underwent freeze-drying process and lyophilization. BSA was then characterized using Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM), and swelling test. The result of characterization using FTIR indicated that amide A, amide B, amide I, amide II, and triple helix of collagen groups were shown in the FTIR graphs. The surface characterization using SEM showed a mean porosity size of 304.866 μm with a porosity proportion of 64%. The swelling analysis showed the proportion of swelling was 1320 times. The BSA has an ideal pore size, functional group and swelling ability for biomaterial candidate in dentistry.