Characterization and production of polyclonal antibody anti excretory secretory protein of Blastocystis sp

Briantono Willy Rendragraha; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Soelih Estoe Pangestie; Heni Puspitasari; Septian Hakim Susantoputro

Characterization and production of polyclonal antibody anti excretory secretory protein of Blastocystis sp

Briantono Willy Rendragraha, Lucia Tri Suwanti, Rahadju Ernawati, Mufasirin Mufasirin, Setiawan Koesdarto, Wiwiek Tyasningsih, Soelih Estoe Pangestie, Heni Puspitasari, Septian Hakim Susantoputro

Research output: Contribution to journal › Article › peer-review

Abstract

This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein profile was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit's serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit.

Original language	English
Pages (from-to)	30-32
Number of pages	3
Journal	Indian Veterinary Journal
Volume	96
Issue number	12
Publication status	Published - 2019

Keywords

Blastocystis sp
Excretory Secretory Protein
Polyclonal Antibody

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title = "Characterization and production of polyclonal antibody anti excretory secretory protein of Blastocystis sp",

abstract = "This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein profile was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit's serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit.",

keywords = "Blastocystis sp, Excretory Secretory Protein, Polyclonal Antibody",

author = "Rendragraha, {Briantono Willy} and Suwanti, {Lucia Tri} and Rahadju Ernawati and Mufasirin Mufasirin and Setiawan Koesdarto and Wiwiek Tyasningsih and Pangestie, {Soelih Estoe} and Heni Puspitasari and Susantoputro, {Septian Hakim}",

year = "2019",

language = "English",

volume = "96",

pages = "30--32",

journal = "Indian Veterinary Journal",

issn = "0019-6479",

publisher = "Indian Veterinary Assocaition",

number = "12",

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TY - JOUR

T1 - Characterization and production of polyclonal antibody anti excretory secretory protein of Blastocystis sp

AU - Rendragraha, Briantono Willy

AU - Suwanti, Lucia Tri

AU - Ernawati, Rahadju

AU - Mufasirin, Mufasirin

AU - Koesdarto, Setiawan

AU - Tyasningsih, Wiwiek

AU - Pangestie, Soelih Estoe

AU - Puspitasari, Heni

AU - Susantoputro, Septian Hakim

PY - 2019

Y1 - 2019

N2 - This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein profile was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit's serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit.

AB - This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein profile was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit's serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit.

KW - Blastocystis sp

KW - Excretory Secretory Protein

KW - Polyclonal Antibody

UR - http://www.scopus.com/inward/record.url?scp=85078163989&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85078163989

SN - 0019-6479

VL - 96

SP - 30

EP - 32

JO - Indian Veterinary Journal

JF - Indian Veterinary Journal

IS - 12

ER -

Characterization and production of polyclonal antibody anti excretory secretory protein of Blastocystis sp

Abstract

Keywords

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