Characterization and Penetration Profile of Resveratrol-loaded Nanostructured Lipid Carrier (NLC) for Topical Anti-aging

Skin aging is an essential factor in the degradation of skin’s structural components, which is signed by less elastic and drier, lines and wrinkles, and looks and feels like older skin. Resveratrol (RSV) is a polyphenol compound with a potent antioxidant and antiaging effect, which can be found in many plants, such as red wine, red grapes, peanuts, berries, and Japanese knotweed root. It is active in inhibiting and neutralizing the formation of ROS and synthetic radicals under in vitro conditions. RSV is an unstable to light compound and insoluble in water, which has to be protected by proper delivery systems such as Nanostructured Lipid Carrier (NLC). This study aimed to determine the characterization and penetration profile of RSV-loaded NLC (RSV-NLC) using the High Shear Homogenization (HSH) method. RSV-NLC was formulated with four different surfactant ratios (5:5; 6:6; 7:7; 8:8) and characterized for entrapment efficiency, organoleptic, viscosity, pH, zeta potential, melting temperature, crystal lattice analysis, particle morphology, particle size, and Polydispersity Index (PI), then examined for their penetration profile. The different ratios of Tween 80 and Span 20 affect the characteristics of RSV-NLC (viscosity, zeta potential, particle morphology, and entrapment efficiency) but do not affect the penetration depth of RSV in the skin. Tween 80:Span 20 (5:5) is the best combination for RSV-NLC in characterization and penetration profile.