Cell free fetal dna [cff-dna]: A non-invasive paternity technique by maternal blood

Background Paternity test to an unborn child by DNA analysis is one of technique to determine whether or not a man is the biological father of a child. The test compares the DNA pattern of a child to that of his or her suspected father to examine for evidence of DNA inheritance that indicates the certainty of a biological relationship. This DNA inheritance is by the lineage of genetic materials from biological parents. A determination is through a theory that naturally in the maternal pregnant body there is a traffic of molecules and even cells from the fetus to the maternal body and vice versa. This is referred to cell-free fetal DNAs technique. To date, paternity tests by means of DNA tests using cell-free fetal DNAs have not been widely demonstrated. Purpose The purpose of this study is to demonstrate the use of cell-free fetal DNAs of the blood of pregnant women as a non-invasive technique for paternity tests at CSFIPO, TPOX, THO1, and vWA. To achieve the purpose, the study implied an observational through crosssectional design. Results Study findings showed that; based on isolation of sample DNAs, the mean level of maternal plasma DNA was 24.70µg/ml. Furthermore, the PCR amplification visualization of CODIS STR loci of CSFIPO, THO1, TPOX, and vWA as well as the positive control (k562) showed the matching alleles. Conclusion DNAs isolated from maternal plasma of second and third trimester pregnant women can be an alternative material in paternity tests, which is a non-invasive technique.