1 Citation (Scopus)


Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE +CD35 +and CFSE +CD89 +was measured and analyzed using flow cytometry. Results The expression of CFSE +CD89 +in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p -value of 0.0001, while the expression of CFSE +CD35 +in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group. Conclusions The expression ratio of CFSE +CD89 +and CFSE +CD35 +constitutes a marker for S-ECC.

Original languageEnglish
Article numberEJD1900349
Pages (from-to)386-392
Number of pages7
JournalEuropean Journal of Dentistry
Issue number3
Publication statusPublished - 1 Jul 2020


  • CD35
  • CD89
  • biomarker
  • caries
  • neutrophils


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