Abstract
Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE +CD35 +and CFSE +CD89 +was measured and analyzed using flow cytometry. Results The expression of CFSE +CD89 +in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p -value of 0.0001, while the expression of CFSE +CD35 +in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group. Conclusions The expression ratio of CFSE +CD89 +and CFSE +CD35 +constitutes a marker for S-ECC.
Original language | English |
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Article number | EJD1900349 |
Pages (from-to) | 386-392 |
Number of pages | 7 |
Journal | European Journal of Dentistry |
Volume | 14 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Jul 2020 |
Keywords
- CD35
- CD89
- biomarker
- caries
- neutrophils