TY - JOUR
T1 - CASES OF MULTIDRUG RESISTANCE (MDR) AND EXTENDED SPECTRUM BETA-LACTAMASE (ESBL) PRODUCING ESCHERICHIA COLI FROM BROILER CHICKEN IN BLITAR, INDONESIA
AU - Wibisono, Freshinta Jellia
AU - Sumiarto, Bambang
AU - Untari, Tri
AU - Effendi, Mustofa Helmi
AU - Permatasari, Dian Ayu
AU - Witaningrum, Adiana Mutamsari
N1 - Funding Information:
This study was funded in part by the Direktorat Riset dan Pengabdian Masyarakat, Deputi Bidang Penguatan Riset dan Pengembangan Kementerian Riset dan Teknologi/ Badan Riset dan Inovasi Nasional, Indonesia in fiscal year 2021; grant number : 418/UN3.15/PT/2021 Ethical clearance : Cloacal swabs were used in this study, hence ethical clearance was not necessary. Cloacal swab samples were collected from Blitar area in East Java province, Indonesia. Conflict of interest : Nil. REFERENCES
Publisher Copyright:
© 2021. All Rights Reserved.
PY - 2021/8
Y1 - 2021/8
N2 - The study was isolated Escherichia coli from cloacal swab of broiler chicken farms in Blitar area to investigate cases of Multidrug Resistance (MDR) Escherichia coli and their Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli. This research was conducted on broiler chicken farms in Blitar district in June until August 2019. Samples using cloaca swabs on broiler chickens were 160 animals from 6 districts in Blitar district. Samples were taken at random and brought to the laboratory for isolation of Escherichia coli bacteria. Positive isolates of Escherichia coli were tested for antibiotic sensitivity and Escherichia coli resistant to beta lactam groups were then confirmed using the Double Disc Synergy Test (DDST) confirmation test, to confirm as ESBL-producing bacteria. The results showed the percentage of antibiotic resistance to Escherichia coli bacteria in broilers in Blitar District was 88.75% (Ampicillin), 78.75% (Streptomycin), 76.87% (Erithromycin), 50.63% (Tetracyclin) and 75% (sulphamethoxazole-trimethoprim). The incidence of Multi-Drug Resistant (MDR) Escherichia coli bacteria in Broiler chickens in Blitar District was 85.63%, and around 28.75% were Escherichia coli bacteria that produce Extended Spectrum Beta-Lactamase (ESBL). In conclusion, the high level of ESBL-producing Escherichia coli in broiler chicken cloaca swabs is a threat to public health and the environment and is an important concern to reduce the rate of its spread.
AB - The study was isolated Escherichia coli from cloacal swab of broiler chicken farms in Blitar area to investigate cases of Multidrug Resistance (MDR) Escherichia coli and their Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli. This research was conducted on broiler chicken farms in Blitar district in June until August 2019. Samples using cloaca swabs on broiler chickens were 160 animals from 6 districts in Blitar district. Samples were taken at random and brought to the laboratory for isolation of Escherichia coli bacteria. Positive isolates of Escherichia coli were tested for antibiotic sensitivity and Escherichia coli resistant to beta lactam groups were then confirmed using the Double Disc Synergy Test (DDST) confirmation test, to confirm as ESBL-producing bacteria. The results showed the percentage of antibiotic resistance to Escherichia coli bacteria in broilers in Blitar District was 88.75% (Ampicillin), 78.75% (Streptomycin), 76.87% (Erithromycin), 50.63% (Tetracyclin) and 75% (sulphamethoxazole-trimethoprim). The incidence of Multi-Drug Resistant (MDR) Escherichia coli bacteria in Broiler chickens in Blitar District was 85.63%, and around 28.75% were Escherichia coli bacteria that produce Extended Spectrum Beta-Lactamase (ESBL). In conclusion, the high level of ESBL-producing Escherichia coli in broiler chicken cloaca swabs is a threat to public health and the environment and is an important concern to reduce the rate of its spread.
KW - Broiler chicken
KW - cloacal swab
KW - ESBL
KW - Escherichia coli
KW - MDR
KW - public health
UR - http://www.scopus.com/inward/record.url?scp=85114858709&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85114858709
SN - 0972-5075
VL - 21
SP - 1923
EP - 1929
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
ER -
