Carbonate hydroxyapatite-hyaluronic acid as bone healing accelerator: In-vitro and in-vivo studies on the alveolar bone of wistar rats

Bone graft materials have been used extensively in periodontics support the success of the periodontal tissue treatment. By merely applying Carbonate hydroxyapatite for the treatment of bone is not the top way to form new bones. Hence, the utilization of regenerative materials such as hyaluronic acid, which is utilized in conjunction with Carbonate hydroxyapatite was expected to increase the success of periodontal treatment. To examine the effect of Carbonate Hydroxyapatite addition to hyaluronic acid 0.02%, post-tooth extraction on the number of osteoblasts and amount of Osteoprotegerin (OPG) and TGF Beta 1 in the Wistar rats' bone defect treatment. This study is constituted laboratory-based experimental research with a posttest-only control group. 27 rats (Rattus norvegicus) represented the animal study model in this research. The sample population was divided into 3 treatment groups; K0: the untreated control group; K1: socket filled with CHA Graft. K2: socket filled with CHA and hyaluronic acid. The K2 group had the highest number of osteoblasts when compared with the control group (±; P < 0.001) and the strongest OPG and TGF-β1 expression of K2 groups (±; P < 0.01). The differences in each group were tested by one way ANOVA test (α = 0.05). The carbonate hydroxyapatite combined with Hyaluronic Acid significantly increased the number of osteoblasts, OPG and TGF-β1in the new bone formations. It is a potential treatment for tissue engineering-based periodontal defect.