Modification of carbon paste electrode (CPE) coated with poly-melamine (PM) and gold nanoparticles (AuNPs) was produced by electrochemical synthesis. The morphological characteristic of PM/AuNPs/CPE was characterized by field emission scanning electron microscope (FESEM), the effect of supporting electrolyte and scan rate were assessed by cyclic voltammetry (CV) to get suitable conditions for hydroquinone (HQ) analysis, and the linear sweep voltammetry (LSV) was used to determine HQ into the optimum condition. The result shows the PM/AuNPs/CPE has been successfully determined the responses of HQ in a presence of Arbutin as interference. The modified electrode also enhances the electrocatalytic activities toward the oxidation peak of hydroquinone (HQ) 5 fold higher than the bare CPE. These modified electrodes displayed linear concentration ranging from 0.4 to 10 μM with detection limit of 0.27 μM. In addition, the PM/AuNPs/CPE shows high selectivity, sensitivity and excellent practice for HQ analysis in the real sample with satisfying recovery.