Abstract
This research aim to optimize the lipase production of Micrococcus sp. isolated from oil palm contaminated soil. The production of lipase enzymes is carried out in modified medium containing olive oil, sea salt, yeast extract with various types of carbon source (glucose, sucrose, glycerol) and nitrogen sources (tryptone, ammonium phosphate, urea and ammonium nitrate). The activity of lipase enzyme was determined by UV-Vis spectrophotometry method toward ρ-nitrophenylpalmitate (ρ-NPP) as a substrate. The results showed that glucose 1% was better carbon source compared with sucrose and glycerol. Ammonium phosphate, urea and ammonium nitrate were good nitrogen sources for lipase production of Micrococcus sp., although tryptone was the best nitrogen source.
| Original language | English |
|---|---|
| Article number | 012029 |
| Journal | IOP Conference Series: Earth and Environmental Science |
| Volume | 217 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 9 Jan 2019 |
| Event | 2nd International Conference on Collaboration Seminar of Chemistry and Industry, CoSCI 2018, in conjunction with 23rd Indonesian Society for Biochemistry and Molecular Biology, ISBMB 2018 - Surabaya, Indonesia Duration: 11 Oct 2018 → 12 Oct 2018 |
Keywords
- Lipase
- Micrococcus sp.
- carbon source
- nitrogen source
- soil